A comparison of extended spectrum β-lactamase producing Escherichia coli from clinical, recreational water and wastewater samples associated in time and location.
A comparison of extended spectrum β-lactamase producing Escherichia coli from clinical, recreational water and wastewater samples associated in time and location.
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DOI：
10.1371/journal.pone.0186576
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Jenum PA
中科院分区：
文献类型：
--
作者：
Jørgensen SB;Søraas AV;Arnesen LS;Leegaard TM;Sundsfjord A;Jenum PA
产生超广谱 β-内酰胺酶的大肠杆菌 (ESBL-EC) 通过废水和污水排泄到环境中，从而重新污染人类和动物。这项观察性研究的目的是检测和量化娱乐用水和废水中的 ESBL-EC，并对环境菌株与地理上相关的人类尿液 ESBL-EC 进行遗传和表型比较分析。对来自四个不同海滩的休闲淡水和咸水样本以及来自附近污水处理厂的废水样本进行过滤，并在差异化和 ESBL 选择性培养基上进行培养。经过抗菌药物敏感性测试和多位点可变串联重复次数测定 (MLVA) 后，通过全基因组测序 (WGS) 对娱乐水中选定的 ESBL-EC 菌株进行了表征，并与生活在同一地区的人们的废水和人尿分离物进行了比较。我们在娱乐水样中检测到 ESBL-EC 的次数为 8/20 (40%)，代表所有地点。 ESBL-EC 与大肠杆菌菌落形成单位总数的比率从 0% 到 3.8% 不等。所有废水样品中都存在 ESBL-EC，比例为 0.56-0.75%。 ST131 在尿液和废水样品中最为常见，而 ST10 在水样品中占主导地位。在所有隔室中均检测到 8 种 ST 和相同的 ESBL-EC MLVA 型。临床 ESBL-EC 分离株更有可能具有多重耐药性 (p<0.001)。这项研究证实，娱乐水域中存在 ESBL-EC，包括那些能够引起人类感染的物质，人类有可能接触这些 ESBL-EC，并随后在沐浴者中进行肠道定植和感染。即使在人类和动物抗生素消费受到严格限制的国家，城市水生环境中也存在多重耐药大肠杆菌菌株。
Extended spectrum β-lactamase producing Escherichia coli (ESBL-EC) are excreted via effluents and sewage into the environment where they can re-contaminate humans and animals. The aim of this observational study was to detect and quantify ESBL-EC in recreational water and wastewater, and perform a genetic and phenotypic comparative analysis of the environmental strains with geographically associated human urinary ESBL-EC. Recreational fresh- and saltwater samples from four different beaches and wastewater samples from a nearby sewage plant were filtered and cultured on differential and ESBL-selective media. After antimicrobial susceptibility testing and multi-locus variable number of tandem repeats assay (MLVA), selected ESBL-EC strains from recreational water were characterized by whole genome sequencing (WGS) and compared to wastewater and human urine isolates from people living in the same area. We detected ESBL-EC in recreational water samples on 8/20 occasions (40%), representing all sites. The ratio of ESBL-EC to total number of E. coli colony forming units varied from 0 to 3.8%. ESBL-EC were present in all wastewater samples in ratios of 0.56–0.75%. ST131 was most prevalent in urine and wastewater samples, while ST10 dominated in water samples. Eight STs and identical ESBL-EC MLVA-types were detected in all compartments. Clinical ESBL-EC isolates were more likely to be multidrug-resistant (p<0.001). This study confirms that ESBL-EC, including those that are capable of causing human infection, are present in recreational waters where there is a potential for human exposure and subsequent gut colonisation and infection in bathers. Multidrug-resistant E. coli strains are present in urban aquatic environments even in countries where antibiotic consumption in both humans and animals is highly restricted.
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影响因子：
5.2
作者：
Bonnedahl, J.;Drobni, P.;Drobni, M.
通讯作者：
Drobni, M.
影响因子：
2.1
作者：
Damborg P;Morsing MK;Petersen T;Bortolaia V;Guardabassi L
通讯作者：
Guardabassi L
影响因子：
9.4
作者：
Joensen, Katrine Grimstrup;Scheutz, Flemming;Aarestrup, Frank M.
通讯作者：
Aarestrup, Frank M.
DOI：
10.5604/12321966.1167710
发表时间：
2015-01-01
影响因子：
1.7
作者：
Cornejova, Tatiana;Venglovsky, Jan;Kmet, Vladimir
通讯作者：
Kmet, Vladimir
影响因子：
3
作者：
Birkett, Christopher I.;Ludlam, Hugo A.;Curran, Martin D.
通讯作者：
Curran, Martin D.