Evaluation of the Immunogenicity in Mice Orally Immunized with Recombinant Lactobacillus casei Expressing Porcine Epidemic Diarrhea Virus S1 Protein.
Evaluation of the Immunogenicity in Mice Orally Immunized with Recombinant Lactobacillus casei Expressing Porcine Epidemic Diarrhea Virus S1 Protein.
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DOI：
10.3390/v14050890
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发表时间：
2022-04-25
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猪流行性腹泻（Porcine epidemic diarrhea，PED）是以腹泻、呕吐和脱水为主要特征的一种急性肠道传染病。该病是由猪流行性腹泻病毒（PEDV）感染肠道粘膜表面引起的。因此，通过口服途径进行粘膜免疫是一种有效的免疫方法。乳酸菌具有耐酸、耐胆盐、提高粘膜免疫力等特性，是口服疫苗的理想载体。PEDV的S1糖蛋白介导病毒与细胞受体的结合并诱导针对病毒的中和抗体。因此，本研究以缺失upp基因型的干酪乳杆菌（Δupp ATCC 393）为出发菌株，筛选表达PEDV S1糖蛋白的重组菌株pPG-SD-S1/Δupp ATCC 393。用已鉴定表达的重组细菌口服免疫小鼠3次，观察70天内抗PEDV IgG和分泌免疫球蛋白A水平的变化。结果表明，口服重组细菌后，抗体水平显着提高。免疫后第42天检测到细胞外细胞因子，表明小鼠产生了高水平的体液和细胞免疫应答。上述结果表明pPG-SD-S1/Δupp ATCC 393作为抗PEDV的口服疫苗具有很大的潜力。
Porcine epidemic diarrhea (PED), characterized by diarrhea, vomiting, and dehydration, is an acute enteric infectious disease of pigs. The disease is caused by porcine epidemic diarrhea virus (PEDV), which infects the intestinal mucosal surface. Therefore, mucosal immunization through the oral route is an effective method of immunization. Lactic acid bacteria, which are acid resistant and bile-salt resistant and improve mucosal immunity, are ideal carriers for oral vaccines. The S1 glycoprotein of PEDV mediates binding of the virus with cell receptors and induces neutralizing antibodies against the virus. Therefore, we reversely screened the recombinant strain pPG-SD-S1/Δupp ATCC 393 expressing PEDV S1 glycoprotein by Lactobacillus casei deficient in upp genotype (Δupp ATCC 393). Mice were orally immunized three times with the recombinant bacteria that had been identified for expression, and the changes of anti-PEDV IgG and secreted immunoglobulin A levels were observed over 70 days. The results indicated that the antibody levels notably increased after oral administration of recombinant bacteria. The detection of extracellular cytokines on the 42nd day after immunization indicated high levels of humoral and cellular immune responses in mice. The above results demonstrate that pPG-SD-S1/Δupp ATCC 393 has great potential as an oral vaccine against PEDV.
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影响因子：
3.3
作者：
Gerdts V;Zakhartchouk A
通讯作者：
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DOI：
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发表时间：
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期刊：
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