Exploiting Illumina sequencing for the development of 95 novel polymorphic EST-SSR markers in common vetch (Vicia sativa subsp. sativa).
Exploiting Illumina sequencing for the development of 95 novel polymorphic EST-SSR markers in common vetch (Vicia sativa subsp. sativa).
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DOI：
10.3390/molecules19055777
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发表时间：
2014-05-05
期刊：
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Liu Z;Liu P;Luo D;Liu W;Wang Y
巢菜（Vicia sativa subsp. sativa）是一种自花授粉的二倍体物种，由于其生长周期短、营养价值高以及可用作干草、谷物、青贮饲料和绿肥等多种用途，是世界上最重要的一年生豆科植物之一。然而，现有的巢菜简单重复序列（SSR）标记不足以满足对这一重要物种进行遗传和分子研究不断发展的需求。在此，我们旨在从巢菜的Illumina转录组中开发并鉴定若干多态性EST - SSR标记。从1025个长度大于1000bp的单基因中总共鉴定出1071个潜在的EST - SSR标记，然后设计并合成了450对引物。最终，针对包括50个个体的10份巢菜材料开发出了95对多态性引物。在95个EST - SSR标记中，等位基因数量从3个到13个不等，多态性信息含量值从0.09到0.98不等。观察到的杂合度值从0.00到1.00不等，预期杂合度值从0.11到0.98不等。从巢菜Illumina转录组开发的这95个EST - SSR标记可极大地促进该物种相关的遗传和分子育种研究的发展。
The common vetch (Vicia sativa subsp. sativa), a self-pollinating and diploid species, is one of the most important annual legumes in the world due to its short growth period, high nutritional value, and multiple usages as hay, grain, silage, and green manure. The available simple sequence repeat (SSR) markers for common vetch, however, are insufficient to meet the developing demand for genetic and molecular research on this important species. Here, we aimed to develop and characterise several polymorphic EST-SSR markers from the vetch Illumina transcriptome. A total number of 1,071 potential EST-SSR markers were identified from 1025 unigenes whose lengths were greater than 1,000 bp, and 450 primer pairs were then designed and synthesized. Finally, 95 polymorphic primer pairs were developed for the 10 common vetch accessions, which included 50 individuals. Among the 95 EST-SSR markers, the number of alleles ranged from three to 13, and the polymorphism information content values ranged from 0.09 to 0.98. The observed heterozygosity values ranged from 0.00 to 1.00, and the expected heterozygosity values ranged from 0.11 to 0.98. These 95 EST-SSR markers developed from the vetch Illumina transcriptome could greatly promote the development of genetic and molecular breeding studies pertaining to in this species.
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使用 Illumina 测序对橡胶树 (Hevea brasiliensis Muell. Arg.) 中的树皮转录组进行从头组装和表征以及 EST-SSR 标记的开发
影响因子：
4.4
作者：
Li D;Deng Z;Qin B;Liu X;Men Z
通讯作者：
Men Z
DOI：
10.3390/molecules18021844
发表时间：
2013-01-31
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Suresh S;Park JH;Cho GT;Lee HS;Baek HJ;Lee SY;Chung JW
通讯作者：
Chung JW
影响因子：
3.7
作者：
Liu Z;Chen T;Ma L;Zhao Z;Zhao PX;Nan Z;Wang Y
通讯作者：
Wang Y
影响因子：
2.9
作者：
Castiglione, M. Ruffini;Frediani, M.;Cremonini, R.
通讯作者：
Cremonini, R.
影响因子：
5.4
作者：
Iniguez-Luy, Federico L.;Voort, Amy V.;Osborn, Thomas C.
通讯作者：
Osborn, Thomas C.