Evaluation of biomarkers of exposure and effect in juvenile areolated grouper (Epinephelus areolatus) on foodborne exposure to benzo[a]pyrene
Evaluation of biomarkers of exposure and effect in juvenile areolated grouper (Epinephelus areolatus) on foodborne exposure to benzo[a]pyrene
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幼年斑斑石斑鱼（Epinephelus areolatus）食源性苯并[a]芘暴露的生物标志物评估和影响
DOI：
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发表时间：
2003
影响因子：
4.1
通讯作者：
P. Lam
中科院分区：
文献类型：
--
作者：
R. Wu;C. Pollino;D. Au;G. Zheng;B. B. H. Yuen;P. Lam
每天向石斑鱼幼鱼施用食源性苯并[a]芘（B[a]P）（Epinealus areolatus）在两个环境现实浓度下的（低剂量0.25 μg B[a]P/g体重/d和高剂量12.5 μg B[a]P/g体重/d），以研究B[a]P、肝乙氧基试卤灵-O-脱乙基酶（EROD）活性、生长、RNA：DNA比率、雌二醇、睾酮和三碘甲状腺原氨酸（T3）。在用B[a]P饮食喂养四周后，用正常饮食喂养鱼另外四周以研究净化期间各种生物标志物的恢复。暴露1周后，大鼠肌肉B[a]P负荷和肝脏EROD活性均显著增加。在暴露的第4周之前，组织中的身体负荷保持稳定，此时高剂量组的浓度显著增加，同时发现EROD降低。在净化期间，身体负荷在第2周降低，EROD在第1周降低。生长和RNA：DNA比例不变。尽管性类固醇水平存在较大差异，但在第四周睾酮水平明显升高，表明B[a]P可能会破坏鱼类性类固醇的平衡。值得注意的是，在暴露的第四周和净化的第一周发现血浆游离T3浓度增加，这表明在慢性暴露期间发育和生殖可能存在潜在风险。我们的数据还表明，这些激素紊乱不是持久的，正常的激素水平可以恢复后不久，污染减轻。
Food‐borne benzo[a]pyrene (B[a]P) was administered daily to juvenile grouper (Epinephelus areolatus) at two environmentally realistic concentrations (a low dose of 0.25 μg B[a]P/g body wt/d and a high dose of 12.5 μg B[a]P/g body wt/d) to investigate and relate temporal changes in body burden of B[a]P, hepatic ethoxyresorufin‐O‐deethylase (EROD) activities, growth, RNA:DNA ratio, estradiol, testosterone, and triiodothyronine (T3). After feeding with B[a]P diets for four weeks, fish were fed with normal diet for another four weeks to study recovery of the various biomarkers during the depuration period. After one week of exposure, both body muscle B[a]P burdens and hepatic EROD activities significantly increased. Body burdens were stable in tissues until the fourth week of exposure, when concentrations in the high‐dose group increased markedly, at which time a concomitant decrease in EROD was found. During the depuration period, body burdens decreased in the second week, and EROD declined in the first week. Growth and RNA:DNA ratio were unaltered. Despite large variations found in sex steroid levels, elevation of testosterone was clearly evident in the fourth week, showing that B[a]P may disrupt the balance of sex steroids in fish. Significantly, increases in plasma‐free T3 concentrations were found in the fourth week of exposure and the first week of depuration, suggesting that development and reproduction may potentially be at risk during chronic exposures. Our data also suggest that these hormonal disturbances are not persistent and that normal hormonal levels can be restored soon after contamination is abated.
影响因子：
10.4
作者：
通讯作者：
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用于研究化学物质对女性生殖内分泌功能影响的硬骨鱼模型。
DOI：
10.1002/jez.1402560421
发表时间：
1990
期刊：
The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry
影响因子：
--
作者：
Thomas,P
通讯作者：
Thomas,P