Gene expression profiles of human adipose tissue-derived mesenchymal stem cells are modified by cell culture density.
Gene expression profiles of human adipose tissue-derived mesenchymal stem cells are modified by cell culture density.
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DOI：
10.1371/journal.pone.0083363
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Koo HH
中科院分区：
文献类型：
--
作者：
Kim DS;Lee MW;Yoo KH;Lee TH;Kim HJ;Jang IK;Chun YH;Kim HJ;Park SJ;Lee SH;Son MH;Jung HL;Sung KW;Koo HH
Previous studies conducted cell expansion ex vivo using low initial plating densities for optimal expansion and subsequent differentiation of mesenchymal stem cells (MSCs). However, MSC populations are heterogeneous and culture conditions can affect the characteristics of MSCs. In this study, differences in gene expression profiles of adipose tissue (AT)-derived MSCs were examined after harvesting cells cultured at different densities. AT-MSCs from three different donors were plated at a density of 200 or 5,000 cells/cm2. After 7 days in culture, detailed gene expression profiles were investigated using a DNA chip microarray, and subsequently validated using a reverse transcription polymerase chain reaction (RT-PCR) analysis. Gene expression profiles were influenced primarily by the level of cell confluence at harvest. In MSCs harvested at ∼90% confluence, 177 genes were up-regulated and 102 genes down-regulated relative to cells harvested at ∼50% confluence (P<0.05, FC>2). Proliferation-related genes were highly expressed in MSCs harvested at low density, while genes that were highly expressed in MSCs harvested at high density (∼90% confluent) were linked to immunity and defense, cell communication, signal transduction and cell motility. Several cytokine, chemokine and growth factor genes involved in immunosuppression, migration, and reconstitution of damaged tissues were up-regulated in MSCs harvested at high density compared with MSCs harvested at low density. These results imply that cell density at harvest is a critical factor for modulating the specific gene-expression patterns of heterogeneous MSCs.
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影响因子：
4.5
作者：
Hwang JH;Shim SS;Seok OS;Lee HY;Woo SK;Kim BH;Song HR;Lee JK;Park YK
通讯作者：
Park YK
影响因子：
--
作者：
Lee, Myoung Woo;Kim, Dae Seong;Koo, Hong Hoe
通讯作者：
Koo, Hong Hoe
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
4
作者：
Braun, Julian;Kurtz, Andreas;Dong, Jun
通讯作者：
Dong, Jun
影响因子：
3.3
作者：
Keyser, Kirsten A.;Beagles, Karen E.;Kiem, Hans-Peter
通讯作者：
Kiem, Hans-Peter