A chip-based immunoaffinity capillary electrophoresis assay for assessing hormones in human biological fluids.
A chip-based immunoaffinity capillary electrophoresis assay for assessing hormones in human biological fluids.
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一种基于芯片的免疫亲和力毛细血管电泳测定法，用于评估人类生物流体中的激素。
DOI：
10.1002/elps.200700785
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发表时间：
2008-08
期刊：
影响因子：
2.9
通讯作者：
Kalish, Heather
中科院分区：
文献类型：
--
作者：
Wellner, Edward F.;Kalish, Heather
A chip-based capillary electrophoresis system has been designed for assessing the concentrations of four hormones in whole human blood, saliva, and urine. The desired analytes were isolated by immunoextraction using a panel of four analyte-specific antibodies immobilized onto a glass fiber insert within the injection port of the chip. Following extraction, the captured analytes were labeled prior to electro-elution into the chip separation channel, where they were resolved into four individual peaks in circa 2 min. Quantification of each peak was achieved by on-line LIF detection and integration of the area under each peak. Comparison to commercial high-sensitivity immunoassays demonstrated that the chip-based assay provided fast, accurate, and precise measurements for the analytes under investigation. As the availability of commercially available antibodies rapidly expands, the application of this system will greatly increase. Chip-based CE separations of multiple analytes from a single sample also provide a significant advantage in the analysis of small samples.
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通讯作者：
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