Co-expression of host and viral microRNAs in porcine dendritic cells infected by the pseudorabies virus.
Co-expression of host and viral microRNAs in porcine dendritic cells infected by the pseudorabies virus.
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DOI：
10.1371/journal.pone.0017374
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发表时间：
2011-03-08
期刊：
影响因子：
3.7
通讯作者：
Giuffra E
中科院分区：
文献类型：
--
作者：
Anselmo A;Flori L;Jaffrezic F;Rutigliano T;Cecere M;Cortes-Perez N;Lefèvre F;Rogel-Gaillard C;Giuffra E
microRNA是小的非编码RNA，大约22 nt长，可以调节动物和植物中的基因表达。在宿主和病原体之间的物理串扰上增加了一层复杂性。树突状细胞（DC）和假病毒（PRV）是猪的甲状腺病毒，在猪中引起甲状腺病，并在猪中造成八个小时的毒po虫。每个样品的大小为21-24个核苷酸，从六个图书馆中获得了三个被感染和模拟感染的DC的生物复制品。先前在专用数据库中注释的猪基因组序列和MiRNA通过序列比对，允许鉴定未知的猪以及由PRV的大潜伏期转录（LLT）转录的五个miRNA。进行病毒miRNA和对不同表达的猪miRNA的Ingenuity途径分析，以使已鉴定的小RNA分子背景化并在功能上表征了它们参与基因表达的转录后调节。表达不同的猪miRNA确定了一个独特的靶基因网络，在神经系统的发展和功能和感染机制的发育和功能中具有非常重要的功能，暗示宿主和病毒miRNA的调节对于建立PRV潜伏期是必不可少的。
MicroRNAs are small non-coding RNAs approximately 22 nt long that modulate gene expression in animals and plants. It has been recently demonstrated that herpesviruses encode miRNAs to control the post-transcriptional regulation of expression from their own genomes and possibly that of their host, thus adding an additional layer of complexity to the physiological cross-talk between host and pathogen. The present study focussed on the interactions between porcine dendritic cells (DCs) and the Pseudorabies virus (PRV), an alpha-herpesvirus causing Aujeszky's disease in pigs. A catalogue of porcine and viral miRNAs, expressed eight hours post-infection, was established by deep sequencing. An average of 2 million reads per sample with a size of 21–24 nucleotides was obtained from six libraries representing three biological replicates of infected and mock-infected DCs. Almost 95% of reads mapped to the draft pig genome sequence and pig miRNAs previously annotated in dedicated databases were detected by sequence alignment. In silico prediction allowed the identification of unknown porcine as well as of five miRNAs transcribed by the Large Latency Transcript (LLT) of PRV. The gene target prediction of the viral miRNAs and the Ingenuity Pathway Analysis of differentially expressed pig miRNAs were conducted to contextualize the identified small RNA molecules and functionally characterize their involvement in the post-transcriptional regulation of gene expression. The results support a role for PRV miRNAs in the maintenance of the host cell latency state through the down-regulation of immediate-early viral genes which is similar to other herpesviruses. The differentially expressed swine miRNAs identified a unique network of target genes with highly significant functions in the development and function of the nervous system and in infectious mechanisms, suggesting that the modulation of both host and viral miRNAs is necessary for the establishment of PRV latency.
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