Genome-Wide Analysis of the Glutathione S-Transferase Gene Family in Capsella rubella: Identification, Expression, and Biochemical Functions.
Genome-Wide Analysis of the Glutathione S-Transferase Gene Family in Capsella rubella: Identification, Expression, and Biochemical Functions.
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DOI：
10.3389/fpls.2016.01325
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发表时间：
2016
影响因子：
5.6
通讯作者：
Lan T
中科院分区：
文献类型：
--
作者：
He G;Guan CN;Chen QX;Gou XJ;Liu W;Zeng QY;Lan T
广泛的亚功能化可以解释为什么在基因复制后保留了这么多基因，这为基因家族扩展提供了引擎。然而，在进化过程中追踪一个超基因家族的进化动力学和功能分歧的特征仍然是一个特别的挑战。在这项研究中，我们从拟南芥的近亲、芥菜科成员风疹芥中鉴定了49个谷胱甘肽s -转移酶（GST）基因。辣椒的gst可分为8类，其中tau和phi的gst数量最多。这两类的扩展主要通过串联基因重复发生，导致染色体上串联排列的基因簇。通过整合辣椒和拟南芥GSTs的系统发育分析、表达模式和生化功能，可以清楚地观察到辣椒（甚至是最近的重复）的同源基因对和拟南芥/油菜的同源GSTs在基因表达和酶特性上的功能差异。这项研究为近缘物种中一个大基因家族的扩展和组织提供了功能证据。
Extensive subfunctionalization might explain why so many genes have been maintained after gene duplication, which provides the engine for gene family expansion. However, it is still a particular challenge to trace the evolutionary dynamics and features of functional divergences in a supergene family over the course of evolution. In this study, we identified 49 Glutathione S-transferase (GST) genes from the Capsella rubella, a close relative of Arabidopsis thaliana and a member of the mustard family. Capsella GSTs can be categorized into eight classes, with tau and phi GSTs being the most numerous. The expansion of the two classes mainly occurs through tandem gene duplication, which results in tandem-arrayed gene clusters on chromosomes. By integrating phylogenetic analysis, expression patterns, and biochemical functions of Capsella and Arabidopsis GSTs, functional divergence, both in gene expression and enzymatic properties, were clearly observed in paralogous gene pairs in Capsella (even the most recent duplicates), and orthologous GSTs in Arabidopsis/Capsella. This study provides functional evidence for the expansion and organization of a large gene family in closely related species.
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影响因子：
12.3
作者：
Dixon DP;Lapthorn A;Edwards R
通讯作者：
Edwards R
影响因子：
4.1
作者：
Benekos, Kostantinos;Kissoudis, Christos;Tsaftaris, Athanasios
通讯作者：
Tsaftaris, Athanasios
DOI：
10.1199/tab.0131
发表时间：
2010-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Dixon, David P;Edwards, Robert
通讯作者：
Edwards, Robert
影响因子：
7.2
作者：
Chen, WQ;Singh, KB
通讯作者：
Singh, KB
影响因子：
7.4
作者：
Chen, Zhong;Gallie, Daniel R.
通讯作者：
Gallie, Daniel R.