A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis.
A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis.
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DOI：
10.1038/s41419-022-05195-9
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发表时间：
2022-09-02
影响因子：
9
通讯作者：
Yu, Xiao-Qiang
中科院分区：
文献类型：
--
作者：
Zhao, Ting;Xiao, Yanhong;Huang, Bo;Ran, Mao-Jiu;Duan, Xin;Wang, Yu-Feng;Lu, Yuzhen;Yu, Xiao-Qiang
在果蝇卵巢中，生态位由体细胞组成，包括终末细丝细胞（tfc）、帽细胞（CCs）和护航细胞（ECs），它们提供维持干细胞更新或启动细胞分化的外部信号。生态位的建立开始于幼虫期终末细丝的形成，但终末细丝发育的潜在机制在很大程度上仍然未知。在这里，我们报道了转录因子纵向缺失（Lola）是卵巢形态发生所必需的。我们发现Lola蛋白在tfc和cc中大量表达，尽管在其他细胞中也有表达，并且在幼虫期建立生态位需要Lola蛋白。重要的是，我们发现lola的敲低表达诱导了成年卵巢的凋亡，并且lola通过抑制cullins 1b的调节因子（Roc1b）的表达来影响成年卵巢的形态发生，Roc1b是一种凋亡相关基因，在精子发生过程中调节caspase的激活。这些发现极大地扩展了我们对果蝇生态位建立和成虫卵发生机制的理解。
In Drosophila ovary, niche is composed of somatic cells, including terminal filament cells (TFCs), cap cells (CCs) and escort cells (ECs), which provide extrinsic signals to maintain stem cell renewal or initiate cell differentiation. Niche establishment begins in larval stages when terminal filaments (TFs) are formed, but the underlying mechanism for the development of TFs remains largely unknown. Here we report that transcription factor longitudinals lacking (Lola) is essential for ovary morphogenesis. We showed that Lola protein was expressed abundantly in TFCs and CCs, although also in other cells, and lola was required for the establishment of niche during larval stage. Importantly, we found that knockdown expression of lola induced apoptosis in adult ovary, and that lola affected adult ovary morphogenesis by suppressing expression of Regulator of cullins 1b (Roc1b), an apoptosis-related gene that regulates caspase activation during spermatogenesis. These findings significantly expand our understanding of the mechanisms controlling niche establishment and adult oogenesis in Drosophila.
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