A novel impedimetric sensor for detecting LAMP amplicons of pathogenic DNA based on magnetic separation
A novel impedimetric sensor for detecting LAMP amplicons of pathogenic DNA based on magnetic separation
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一种基于磁分离检测致病性DNA LAMP扩增子的新型阻抗传感器
DOI：
10.1016/j.snb.2019.127051
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发表时间：
2019-12
期刊：
影响因子：
--
通讯作者：
Yibin Ying
中科院分区：
文献类型：
--
作者：
Sumaira Sharif;Yixian Wang;Zunzhong Ye;Zhen Wang;Qimin Qiu;Shengna Ying;Yibin Ying
结合微流控系统和磁分离技术，研制了一种新型阻抗传感器，用于检测多种食源性致病菌的环介导等温扩增（LAMP）扩增子。LAMP反应的扩增核酸产物（扩增子）可以通过静电吸附过程吸附在磁珠表面上。将所得核酸-磁珠复合物重新分散在去离子水中，并通过将核酸-磁珠复合物注入微流控系统中来测量阻抗响应。由于去离子水的电导率很低（1 ~ 2 μS cm-1），用阻抗法可以检测到微弱的电荷变化。因此，复合材料中核酸所带的大量负电荷可使阻抗信号明显减弱。基于该原理，将该阻抗传感方法应用于多种食源性致病菌的检测，包括大肠杆菌O 157：H7（E. colio 157：H7）、副溶血弧菌（V. parahaemolyticus）、金黄色葡萄球菌（S. aureus）、单核细胞增生李斯特菌（Listeria monocytogenes（L.单核细胞增多症），其实现了低检测限（10个拷贝）。与传统的凝胶电泳方法相比，该方法灵敏度更高，检测速度更快，具有快速、灵敏检测多种靶点的LAMP产物的潜力。
A novel impedimetric sensor was developed for the detection of loop mediated isothermal amplification (LAMP) amplicons of various foodborne pathogens through combining with a microfluidic system and magnetic separation procedure. The amplified nucleic acid products (amplicons) of the LAMP reaction can adsorb on magnetic beads surface via electrostatic adsorption process. The obtained nucleic acid-magnetic bead composites were re-dispersed in deionized water and the impedance response was measured by injecting the nucleic acid-magnetic bead composites into the microfluidic system. On account of the quite low conductivity (1˜2 μS cm―1) of deionized water, the weak change of charge can be detected by impedance. Thus the abundant negative charges of nucleic acids in the composites can produce an obviously reduction of impedance signal. Based on the principle, the developed impedimetric sensing method was used for sensing various foodborne pathogens includingEscherichia coliO157:H7 (E. coliO157:H7),Vibrio parahaemolyticus(V. parahaemolyticus),Staphylococcus aureus(S. aureus), andListeria monocytogenes(L. monocytogenes), which achieved a low detection limit (10 copies). Compared with the conventional gel electrophoresis method, our method is more sensitive and faster, which has a potential for fast and sensitive detection of the LAMP products of various targets.
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影响因子：
7.4
作者：
Shunbi Xie;Yali Yuan;Y. Chai;R. Yuan
通讯作者：
Shunbi Xie;Yali Yuan;Y. Chai;R. Yuan
影响因子：
6.8
作者：
Gulshan Singh;P. Vajpayee;Neetika Rani;Anurag Jyoti;K. Gupta;R. Shanker
通讯作者：
Gulshan Singh;P. Vajpayee;Neetika Rani;Anurag Jyoti;K. Gupta;R. Shanker
影响因子：
11.8
作者：
Scallan E;Hoekstra RM;Angulo FJ;Tauxe RV;Widdowson MA;Roy SL;Jones JL;Griffin PM
通讯作者：
Griffin PM
追踪 DNA 扩增过程中产生的磷酸根离子及其在等温扩增产物视觉检测中的简单用途
影响因子：
4.9
作者：
Zhang, Fang;Wang, Rui;Ying, Yibin
通讯作者：
Ying, Yibin
影响因子：
4.2
作者：
Ahmed, Minhaz Uddin;Nahar, Sharifun;Zourob, Mohammed
通讯作者：
Zourob, Mohammed