A subgroup of MATE transporter genes regulates hypocotyl cell elongation in Arabidopsis.
A subgroup of MATE transporter genes regulates hypocotyl cell elongation in Arabidopsis.
复制标题
MATE 转运蛋白基因的一个亚组调节拟南芥下胚轴细胞的伸长。
DOI：
10.1093/jxb/erv344
复制
发表时间：
2015-07
影响因子：
6.9
通讯作者：
Yu, Fei
中科院分区：
文献类型：
--
作者：
Shao, Jingxia;Qi, Yafei;An, Lijun;Yu, Fei
高等植物的生长受到复杂的管理，以确保在不断变化的环境下制定发展方案。为了剖析这些调控电路，我们对具有改变枝条发育的拟南芥异常枝条(Abs)突变体进行了遗传筛选。在这里，我们报告了两个显性突变体，abb3-1D和abb4-1D，通过激活标签分离。两个突变体均表现出顶端显性表型的“浓密”缺失。ABS3和ABS4分别编码两个密切相关的多药和有毒化合物挤出(Mate)外排转运蛋白家族。ABS3和ABS4以及两个相关的配对基因ABS3L1(ABS3L1)和ABS3L2显示出不同的组织表达谱，但它们的基因产物都定位于晚期内体/前囊泡(LE/PVC)。这四个基因的过度表达分别导致了下胚轴细胞在光照下的伸长受到抑制。另一方面，四重敲除突变体(Mateq)在光照下表现出增强的下胚轴细胞伸长的相反表型。下胚轴细胞在黑暗中的伸长和去黄化过程也受到这些基因突变的影响。在黑暗条件下，外源蔗糖处理减弱了Ab3-1D和Ab4-1D对下胚轴伸长的抑制作用，增强了长时间黑暗处理下Mateq的下胚轴伸长。我们确定ABS3在基因上与感光基因光敏色素B(PHYB)相互作用。我们的结果表明，ABS3和相关的Mate家族转运蛋白是下胚轴细胞伸长的潜在负调控因子，并支持内膜系统，特别是LE/PVC系统与植物细胞伸长调控之间的功能联系。
The growth of higher plants is under complex regulation to ensure the elaboration of developmental programmes under a changing environment. To dissect these regulatory circuits, we carried out genetic screens for Arabidopsis abnormal shoot (abs) mutants with altered shoot development. Here, we report the isolation of two dominant mutants, abs3-1D and abs4-1D, through activation tagging. Both mutants showed a 'bushy' loss of apical dominance phenotype. ABS3 and ABS4 code for two closely related putative Multidrug and Toxic Compound Extrusion (MATE) family of efflux transporters, respectively. ABS3 and ABS4, as well as two related MATE genes, ABS3-Like1 (ABS3L1) and ABS3L2, showed diverse tissue expression profiles but their gene products all localized to the late endosome/prevacuole (LE/PVC) compartment. The over-expression of these four genes individually led to the inhibition of hypocotyl cell elongation in the light. On the other hand, the quadruple knockout mutant (mateq) showed the opposite phenotype of an enhanced hypocotyl cell elongation in the light. Hypocotyl cell elongation and de-etiolation processes in the dark were also affected by the mutations of these genes. Exogenously applied sucrose attenuated the inhibition of hypocotyl elongation caused by abs3-1D and abs4-1D in the dark, and enhanced the hypocotyl elongation of mateq under prolonged dark treatment. We determined that ABS3 genetically interacts with the photoreceptor gene PHYTOCHROME B (PHYB). Our results demonstrate that ABS3 and related MATE family transporters are potential negative regulators of hypocotyl cell elongation and support a functional link between the endomembrane system, particularly the LE/PVC, and the regulation of plant cell elongation.
登录
查看更多内容
影响因子：
21.3
作者：
通讯作者：
--
DOI：
10.1105/tpc.107.054965
发表时间：
2008-07
期刊：
The Plant Cell Online
影响因子：
--
作者：
Fei Yu;Xiayan Liu;M. Alsheikh;Sungsoon Park;S. Rodermel
通讯作者：
Fei Yu;Xiayan Liu;M. Alsheikh;Sungsoon Park;S. Rodermel
影响因子：
9.4
作者：
Pelletier, Sandra;Van Orden, Juergen;Hoefte, Herman
通讯作者：
Hoefte, Herman
DOI：
10.1111/j.1365-313x.2003.02014.x
发表时间：
2004-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Fei Yu;Sungsoon Park;S. Rodermel
通讯作者：
Fei Yu;Sungsoon Park;S. Rodermel
影响因子：
64.8
作者：
Lucio Fregonese
通讯作者：
Lucio Fregonese