Identification and expression characterization of the Phloem Protein 2 (PP2) genes in ramie (Boehmeria nivea L. Gaudich).

Identification and expression characterization of the Phloem Protein 2 (PP2) genes in ramie (Boehmeria nivea L. Gaudich).
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苎麻 (Boehmeria nivea L. Gaudich) 韧皮部蛋白 2 (PP2) 基因的鉴定和表达特征

DOI:
10.1038/s41598-018-28953-2
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发表时间:
2018-07-16
期刊:
影响因子:
4.6
通讯作者:
Wang B
Wang B
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Guo P;Zheng Y;Peng D;Liu L;Dai L;Chen C;Wang B

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韧皮部蛋白2(PP2)是筛选元件和伴生细胞中含量最丰富、最神秘的蛋白质之一,在高等植物的形态维持、同化物运输和伤害保护等方面发挥着重要作用,但到目前为止,还没有鉴定到PP2(BnPP2)基因。在此,共鉴定了15个全长BnPP2基因。这些BnPP2基因对非生物胁迫表现出不同的响应。有趣的是,BnPP2基因对害虫比对其他胁迫更敏感。对BnPP2-15启动子的研究表明,pBnPP2-15可以驱动GUS在叶柄、根和雄蕊中的特异表达,也可以在机械伤害和蚜虫侵染的情况下被诱导。亚细胞定位结果表明,GFP-BnPP2-1、GFP-BnPP2-6、GFP-BnPP2-7、GFP-BnPP2-9、GFP-BnPP2-11和GFP-BnPP2-12主要定位于细胞质。这些结果为阐明BnPP2基因在苎麻中的功能提供了有用的信息。
Phloem protein 2 (PP2) is one of the most abundant and enigmatic proteins in sieve elements and companion cells, which play important roles in the maintenance of morphology, photoassimilate transportation and wound protection in higher plants, but to date, no PP2 (BnPP2) genes had been identified in ramie. Here, a total of 15 full-length BnPP2 genes were identified. These BnPP2 genes exhibited different responses to abiotic stresses. Interestingly, the BnPP2 genes are more sensitive to insect pests than to other stresses. A study of the BnPP2-15 promoter revealed that pBnPP2-15 could drive specific GUS expression in the petiole, root and stamen and could also be induced by mechanical wounding and aphid infection in transgenic Arabidopsis lines. The subcellular localization of six BnPP2 proteins showed that GFP-BnPP2-1, GFP-BnPP2-6, GFP-BnPP2-7, GFP-BnPP2-9, GFP-BnPP2-11 and GFP-BnPP2-12 were predominantly located in the cytoplasm. These results provide useful information elucidating the functions of BnPP2 genes in ramie.
DOI: 10.3390/ijms16023493
发表时间: 2015-02-04
影响因子: 5.6
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An X;Chen J;Zhang J;Liao Y;Dai L;Wang B;Liu L;Peng D
通讯作者: Peng D
DOI: 10.1007/s12041-016-0622-5
发表时间: 2016-03-01
影响因子: 1.5
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DOI: 10.1105/tpc.10.1.35
发表时间: 1998-01-01
期刊: PLANT CELL
影响因子: 11.6
作者:
Knoblauch, M;van Bel, AJE
通讯作者: van Bel, AJE
DOI: 10.1007/s004250050441
发表时间: 1998-11-01
期刊: PLANTA
影响因子: 4.3
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DOI: 10.1073/pnas.97.7.3718
发表时间: 2000-03-28
影响因子: 11.1
作者:
Cutler, SR;Ehrhardt, DW;Somerville, CR
通讯作者: Somerville, CR