Transcriptome profiling and identification of transcription factors in ramie (Boehmeria nivea L. Gaud) in response to PEG treatment, using illumina paired-end sequencing technology.
Transcriptome profiling and identification of transcription factors in ramie (Boehmeria nivea L. Gaud) in response to PEG treatment, using illumina paired-end sequencing technology.
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DOI：
10.3390/ijms16023493
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发表时间：
2015-02-04
影响因子：
5.6
通讯作者：
Peng D
中科院分区：
文献类型：
--
作者：
An X;Chen J;Zhang J;Liao Y;Dai L;Wang B;Liu L;Peng D
苎麻(Boehmeria nivea L.Gaud)俗称中国草，为荨麻科多年生韧皮纤维植物。在中国，苎麻种植、工业和贸易为大约500万人提供了收入。干旱胁迫严重影响了苎麻茎的生长，导致苎麻纤维产量急剧下降。有必要提高苎麻对干旱胁迫的耐受性。然而，目前对苎麻干旱胁迫的调控机制尚不清楚。聚乙二醇水胁迫是评价植物抗旱性的一种常用而简便的方法。在这项研究中，利用Illumina Pair-end测序技术对经过聚乙二醇化处理的苎麻的cdna收集物进行了转录组分析，产生了1.7亿个原始序列读数。在处理24(L2和R2)和72(L3和R3)h的叶片和根之间，有16,798个基因的差异表达(9281个在叶片中，8627个在根中)。其中，AP2(3)、MYB(6)、NAC(9)、锌指(5)和bZIP(2)家族的25个转录因子被认为与干旱胁迫有关。鉴定出的抗旱因子可用于进一步研究苎麻的干旱适应性。
Ramie (Boehmeria nivea L. Gaud), commonly known as China grass, is a perennial bast fiber plant of the Urticaceae. In China, ramie farming, industry, and trade provide income for about five million people. Drought stress severely affects ramie stem growth and causes a dramatic decrease in ramie fiber production. There is a need to enhance ramie’s tolerance to drought stress. However, the drought stress regulatory mechanism in ramie remains unknown. Water stress imposed by polyethylene glycol (PEG) is a common and convenient method to evaluate plant drought tolerance. In this study, transcriptome analysis of cDNA collections from ramie subjected to PEG treatment was conducted using Illumina paired-end sequencing, which generated 170 million raw sequence reads. Between leaves and roots subjected to 24 (L2 and R2) and 72 (L3 and R3) h of PEG treatment, 16,798 genes were differentially expressed (9281 in leaves and 8627 in roots). Among these, 25 transcription factors (TFs) from the AP2 (3), MYB (6), NAC (9), zinc finger (5), and bZIP (2) families were considered to be associated with drought stress. The identified TFs could be used to further investigate drought adaptation in ramie.
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