Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish.
Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish.
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DOI：
10.1038/s41598-018-20922-z
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发表时间：
2018-02-09
影响因子：
4.6
通讯作者：
Tyler CR
中科院分区：
文献类型：
--
作者：
Green JM;Lange A;Scott A;Trznadel M;Wai HA;Takesono A;Brown AR;Owen SF;Kudoh T;Tyler CR
雌激素在一系列发育过程中起着重要作用，暴露于雌激素模拟化学品与野生动物和人类的各种不良健康影响有关。雌激素化学品通常以混合物的形式存在于环境中，可能具有累加效应，但风险分析通常针对单一化学品进行，很少或根本不考虑动物的接触史。在这里，我们开发了一种转基因斑马鱼与光转换荧光团（Kaede，绿色红色的紫外线曝光）在皮肤色素的突变元件（ERE）-Kaede-Casper模型，并将其应用于量化组织特异性的荧光生物传感器响应的组合，雌激素暴露在生命早期使用荧光显微镜和图像分析。我们确定了在生命早期（0-5 dpf）暴露的组织特异性对炔雌醇（EE 2）的敏感性窗口，并说明在0-48 hpf期间暴露于雌激素（EE 2）增强了对不同环境雌激素（EE 2，染料木素和双酚A）的反应性（敏感性），用于随后在发育期间的暴露。我们的研究结果说明了一个有机体的发展阶段和雌激素暴露史的重要性，评估和可能的健康风险与雌激素暴露。
Estrogen plays fundamental roles in a range of developmental processes and exposure to estrogen mimicking chemicals has been associated with various adverse health effects in both wildlife and human populations. Estrogenic chemicals are found commonly as mixtures in the environment and can have additive effects, however risk analysis is typically conducted for single-chemicals with little, or no, consideration given for an animal’s exposure history. Here we developed a transgenic zebrafish with a photoconvertable fluorophore (Kaede, green to red on UV light exposure) in a skin pigment-free mutant element (ERE)-Kaede-Casper model and applied it to quantify tissue-specific fluorescence biosensor responses for combinations of estrogen exposures during early life using fluorescence microscopy and image analysis. We identify windows of tissue-specific sensitivity to ethinylestradiol (EE2) for exposure during early-life (0–5 dpf) and illustrate that exposure to estrogen (EE2) during 0–48 hpf enhances responsiveness (sensitivity) to different environmental estrogens (EE2, genistein and bisphenol A) for subsequent exposures during development. Our findings illustrate the importance of an organism’s stage of development and estrogen exposure history for assessments on, and possible health risks associated with, estrogen exposure.
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DOI：
10.1016/j.bbagrm.2014.06.005
发表时间：
2015-02
影响因子：
4.7
作者：
Bondesson, Maria;Hao, Ruixin;Lin, Chin-Yo;Williams, Cecilia;Gustafsson, Jan-Ake
通讯作者：
Gustafsson, Jan-Ake
影响因子：
11.4
作者：
Coe, Tobias S.;Soeffker, Marta K.;Tyler, Charles R.
通讯作者：
Tyler, Charles R.
影响因子：
14.8
作者：
Hatta, Kohei;Tsujii, Hitomi;Omura, Tomomi
通讯作者：
Omura, Tomomi
影响因子：
64.8
作者：
GIGUERE, V;YANG, N;EVANS, RM
通讯作者：
EVANS, RM
影响因子：
4
作者：
Kortenkamp, Andreas
通讯作者：
Kortenkamp, Andreas