Comprehensive locomotor outcomes correlate to hyperacute diffusion tensor measures after spinal cord injury in the adult rat.
Comprehensive locomotor outcomes correlate to hyperacute diffusion tensor measures after spinal cord injury in the adult rat.
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DOI：
10.1016/j.expneurol.2011.11.015
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发表时间：
2012-05
影响因子：
5.3
通讯作者：
Magnuson, David S. K.
中科院分区：
文献类型：
--
作者：
Kim, Joong H.;Song, Sheng-Kwei;Burke, Darlene A.;Magnuson, David S. K.
关键词：
在成年大鼠中，挫伤性胸脊髓损伤（SCI）后的运动障碍主要是由震中白质损失/功能障碍引起的。这种丧失/功能障碍减少了来自脑和颈脊髓的下行输入，减少了长本体脊髓、脊髓小脑和体感通路等的上行信号。由于脊髓休克会导致暂时性弛缓性麻痹和反射丧失，在不了解损伤严重程度的情况下，很难对挫伤的长期功能后果进行急性预测。现在众所周知，高质量后肢行走的恢复只需要12-15%的震中白质，但前肢-后肢协调和精确行走（网格或水平阶梯）需要更多的跨挫伤沟通。为了将我们对大鼠功能恢复的神经基质的理解转化为临床领域，需要共同的结果测量和成像方式。在目前的研究中，我们进一步探索了其中一种方法，即弥散张量磁共振成像（DTI），这是一种在临床研究中常用的脑成像技术，但很少用于脊髓损伤的诊断或预后。在成年脊髓损伤大鼠模型中，我们发现损伤中心外侧和腹侧白质的超急性（损伤后<3小时）DTI可以预测损伤后4周的电生理和行为（运动）恢复，尽管存在弛缓性麻痹/脊髓休克。脑白质轴向扩散率在3小时时最低为1.5μm2/ms的脑白质区域在4周时能够传导动作电位，侧索内的轴向扩散率在4周时能够高度预测运动功能。这些观察结果表明，急性DTI应该有助于在临床上预测脊髓挫伤后白质的功能。
In adult rats, locomotor deficits following a contusive thoracic spinal cord injury (SCI) are caused primarily by white matter loss/dysfunction at the epicenter. This loss/dysfunction decreases descending input from the brain and cervical spinal cord, and decreases ascending signals in long propriospinal, spinocerebellar and somatosensory pathways, among many others. Predicting the long-term functional consequences of a contusive injury acutely, without knowledge of the injury severity is difficult due to the temporary flaccid paralysis and loss of reflexes that accompanies spinal shock. It is now well known that recovery of high quality hindlimb stepping requires only 12-15% spared white matter at the epicenter, but that forelimb-hindlimb coordination and precision stepping (grid or horizontal ladder) requires substantially more trans-contusion communication. In order to translate our understanding of the neural substrates for functional recovery in the rat to the clinical arena, common outcome measures and imaging modalities are required. In the current study we furthered the exploration of one of these approaches, diffusion tensor magnetic resonance imaging (DTI), a technique now used commonly to image the brain in clinical research but rarely used diagnostically or prognostically for spinal cord injury. In the adult rat model of SCI, we found that hyper-acute (<3 hours post-injury) DTI of the lateral and ventral white matter at the injury epicenter was predictive of both electrophysiological and behavioral (locomotor) recovery at 4 weeks post-injury, despite the presence of flaccid paralysis/spinal shock. Regions of white matter with a minimum axial diffusivity of 1.5μm2/ms at 3 hours were able to conduct action potentials at 4 weeks, and axial diffusivity within the lateral funiculus was highly predictive of locomotor function at 4 weeks. These observations suggest that acute DTI should be useful to provide functional predictions for spared white matter following contusive spinal cord injuries clinically.
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影响因子：
4.2
作者：
Loy, David N.;Kim, Joong Hee;Song, Sheng-Kwei
通讯作者：
Song, Sheng-Kwei
影响因子：
4.2
作者：
Magnuson DS;Smith RR;Brown EH;Enzmann G;Angeli C;Quesada PM;Burke D
通讯作者：
Burke D
影响因子：
4.2
作者：
Kim, Joong H.;Loy, David N.;Song, Sheng-Kwei
通讯作者：
Song, Sheng-Kwei
影响因子：
5.3
作者：
Loy, DN;Magnuson, DSK;Whittemore, SR
通讯作者：
Whittemore, SR
影响因子：
4.2
作者：
Hamers, FPT;Koopmans, GC;Joosten, EAJ
通讯作者：
Joosten, EAJ