Separation of plasmacytoid dendritic cells from B-cell-biased lymphoid progenitor (BLP) and Pre-pro B cells using PDCA-1.
Separation of plasmacytoid dendritic cells from B-cell-biased lymphoid progenitor (BLP) and Pre-pro B cells using PDCA-1.
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DOI：
10.1371/journal.pone.0078408
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shapiro VS
中科院分区：
文献类型：
--
作者：
Medina KL;Tangen SN;Seaburg LM;Thapa P;Gwin KA;Shapiro VS
B细胞偏向性淋巴祖细胞（BLPs）和前原B细胞处于B细胞特化和定型之间的关键节点。然而，这两种细胞群都是异质的，这阻碍了对淋巴祖细胞向B细胞谱系定型时发生的分子变化的研究。在此，我们证明存在与BLPs和前原B细胞共同纯化的PDCA - 1⁺Siglec H⁺浆细胞样树突状细胞（pDCs），它们很少或不表达CD11c或Ly6C。去除PDCA - 1⁺ pDCs可将在BLP和前原B细胞群中表达高水平Rag1 - GFP报告基因的B细胞祖细胞与Rag1 - GFPlow/neg pDCs区分开来。对Flt3配体敲除小鼠和IL - 7Rα敲除小鼠的分析表明，在全淋巴祖细胞（ALP）阶段B细胞发育受阻，因为在BLP或前原B门内的大多数细胞是PDCA - 1⁺ pDCs。因此，去除PDCA - 1⁺ pDCs对于分析BLP和前原B细胞群至关重要。对B220⁺CD19⁻组分中B细胞潜能的分析表明，AA4.1⁺Ly6D⁺PDCA - 1⁻前原B细胞高频产生CD19⁺ B细胞，而该组分中的PDCA - 1⁺ pDCs则不会。有趣的是，共同淋巴祖细胞（CLPs）中PDCA - 1⁺ pDCs的存在可能有助于解释关于这些细胞起源的相互矛盾的结果。
B-cell-biased lymphoid progenitors (BLPs) and Pre-pro B cells lie at a critical juncture between B cell specification and commitment. However, both of these populations are heterogenous, which hampers investigation into the molecular changes that occur as lymphoid progenitors commit to the B cell lineage. Here, we demonstrate that there are PDCA-1+Siglec H+ plasmacytoid dendritic cells (pDCs) that co-purify with BLPs and Pre-pro B cells, which express little or no CD11c or Ly6C. Removal of PDCA-1+ pDCs separates B cell progenitors that express high levels of a Rag1-GFP reporter from Rag1-GFPlow/neg pDCs within the BLP and Pre-pro B populations. Analysis of Flt3-ligand knockout and IL-7Rα knockout mice revealed that there is a block in B cell development at the all-lymphoid progenitor (ALP) stage, as the majority of cells within the BLP or Pre-pro B gates were PDCA-1+ pDCs. Thus, removal of PDCA-1+ pDCs is critical for analysis of BLP and Pre-pro B cell populations. Analysis of B cell potential within the B220+CD19− fraction demonstrated that AA4.1+Ly6D+PDCA-1− Pre-pro B cells gave rise to CD19+ B cells at high frequency, while PDCA-1+ pDCs in this fraction did not. Interestingly, the presence of PDCA-1+ pDCs within CLPs may help to explain the conflicting results regarding the origin of these cells.
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DOI：
10.1084/jem.20020045
发表时间：
2002-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gilliet M;Boonstra A;Paturel C;Antonenko S;Xu XL;Trinchieri G;O'Garra A;Liu YJ
通讯作者：
Liu YJ
DOI：
10.4049/jimmunol.0904203
发表时间：
2010-12-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gwin K;Frank E;Bossou A;Medina KL
通讯作者：
Medina KL
影响因子：
15.3
作者：
Allman, D;Li, J;Hardy, RR
通讯作者：
Hardy, RR
影响因子：
20.3
作者：
Mansson, Robert;Zandi, Sasan;Sigvardsson, Mikael
通讯作者：
Sigvardsson, Mikael
影响因子：
4.4
作者：
Choi, Jinwook;Ko, Myunggon;Seong, Rho H.
通讯作者：
Seong, Rho H.