Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.).
Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.).
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DOI：
10.1007/s00425-009-0989-0
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发表时间：
2009-09
期刊：
影响因子：
4.3
通讯作者：
Inoue K
中科院分区：
文献类型：
--
作者：
Ikegami A;Akagi T;Potter D;Yamada M;Sato A;Yonemori K;Kitajima A;Inoue K
柿（Diospyros kaki Thunb.）在发育的早期阶段积累大量的原花青素（PA）。涩（A）型果实即使在完全成熟后仍富含可溶性多胺，而非涩（NA）型果实在完全成熟前失去这些化合物。作为第一步，以阐明PA积累在这个非模式物种的机制，我们使用抑制性消减杂交，以确定转录积累不同的幼果的A-和NA-型。有趣的是，在A-NA文库中仅鉴定出少数涉及PA生物合成的克隆。以多个克隆为代表的是编码新的1-Cys过氧化物氧还蛋白和家族1糖基转移酶的新成员的那些。定量RT-PCR分析证实了相关的量的PA和积累的转录本编码这些蛋白质在年轻的柿子果实。此外，新的家族1糖基转移酶在大肠杆菌中产生，并显示出有效地催化半乳糖基化在3-羟基的几个花青素和黄酮醇。这些结果表明，柿子果实中PA积累的复杂机制。本文的在线版本（doi：10.1007/s 00425 -009-0989-0）包含补充材料，可供授权用户使用。
Fruits of persimmon (Diospyros kaki Thunb.) accumulate large amounts of proanthocyanidins (PAs) in the early stages of development. Astringent (A)-type fruits remain rich in soluble PAs even after they reach full-mature stage, whereas non-astringent (NA)-type fruits lose these compounds before full maturation. As a first step to elucidate the mechanism of PA accumulation in this non-model species, we used suppression subtractive hybridization to identify transcripts accumulating differently in young fruits of A- and NA-type. Interestingly, only a few clones involved in PA biosynthesis were identified in A–NA libraries. Represented by multiple clones were those encoding a novel 1-Cys peroxiredoxin and a new member of family 1 glycosyltransferases. Quantitative RT-PCR analyses confirmed correlation of the amount of PAs and accumulation of transcripts encoding these proteins in young persimmon fruits. Furthermore, the new family 1 glycosyltransferase was produced in Escherichia coli and shown to efficiently catalyze galactosylation at 3-hydroxyl groups of several anthocyanidins and flavonols. These findings suggest a complex mechanism of PA accumulation in persimmon fruits. The online version of this article (doi:10.1007/s00425-009-0989-0) contains supplementary material, which is available to authorized users.
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影响因子：
12.3
作者：
Dixon DP;Lapthorn A;Edwards R
通讯作者：
Edwards R
影响因子：
3.9
作者：
Kubo, A;Arai, Y;Yoshikawa, T
通讯作者：
Yoshikawa, T
影响因子：
2.9
作者：
GUAN, KL;DIXON, JE
通讯作者：
DIXON, JE
影响因子：
8.1
作者：
Gu, Hai-Feng;Li, Chun-Mei;Wan, Qiong-hong
通讯作者：
Wan, Qiong-hong
影响因子：
4.2
作者：
Gu, LW;Kelm, MA;Prior, RL
通讯作者：
Prior, RL