Effects of Dietary Antimicrobial Growth Promoters on Performance Parameters and Abundance and Diversity of Broiler Chicken Gut Microbiome and Selection of Antibiotic Resistance Genes.
Effects of Dietary Antimicrobial Growth Promoters on Performance Parameters and Abundance and Diversity of Broiler Chicken Gut Microbiome and Selection of Antibiotic Resistance Genes.
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DOI：
10.3389/fmicb.2022.905050
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
抗微生物生长促进剂（AGPs）通常用于肉鸡生产。它们的使用及其对食用动物抗菌素耐药性（AMR）扩散和传播到人类或环境的贡献引起了巨大的社会关注。然而，缺乏关于它们对家禽生产和AMR耐药组影响的综合实验数据。在这里，我们研究了在印度次大陆和世界不同地区的商业肉鸡生产中使用的五种抗菌生长促进剂（维吉尼亚霉素、金霉素、杆菌肽甲基二磺酸盐、林可霉素和泰乐菌素）连续三个生产周期对性能变量的影响，以及使用非培养方法对肠道细菌、噬菌体和耐药基因组特征的影响。在生产周期结束时，AGP对累积生长或饲料效率参数无显著影响，各组的累积死亡率也相似。无论是否补充AGP，许多抗生素耐药基因（ARG）在鸡肠道中普遍存在。总共检测到来自15种抗菌剂类别的62种ARG。AGP的补充影响了几类ARG的选择;然而，这不一定与AGP药物类别相关的基因相关;一些AGP倾向于选择与抗菌剂相关的ARG，而与AGP结构无关。AGPs没有影响肠道细菌群落结构，包括α或β多样性，只有16-20个细菌的操作分类单位（OTU）被显著改变。然而，几种AGP显着降低了一些潜在致病细菌属的种群密度，例如大肠杆菌。金霉素使噬菌体的丰度增加，而其他AGP对噬菌体的丰度影响不显著。考虑到有证据表明，家禽生产中使用的AGP可以选择对一种以上抗菌剂耐药性的耐药性，而且它们对生产性能的影响并不显著，因此需要减少其使用，并需要监测肉鸡养殖场中ARG的传播。
Antimicrobial growth promoters (AGPs) are commonly used in broiler production. There is a huge societal concern around their use and their contribution to the proliferation of antimicrobial resistance (AMR) in food-producing animals and dissemination to humans or the environment. However, there is a paucity of comprehensive experimental data on their impact on poultry production and the AMR resistome. Here, we investigated the effect of five antimicrobial growth promoters (virginiamycin, chlortetracycline, bacitracin methyl disalicylate, lincomycin, and tylosin) used in the commercial broiler production in the Indian subcontinent and in the different parts of the world for three consecutive production cycles on performance variables and also the impact on gut bacteria, bacteriophage, and resistome profile using culture-independent approaches. There was no significant effect of AGPs on the cumulative growth or feed efficiency parameters at the end of the production cycles and cumulative mortality rates were also similar across groups. Many antibiotic resistance genes (ARGs) were ubiquitous in the chicken gut irrespective of AGP supplementation. In total, 62 ARGs from 15 antimicrobial classes were detected. Supplementation of AGPs influenced the selection of several classes of ARGs; however, this was not correlated necessarily with genes relevant to the AGP drug class; some AGPs favored the selection of ARGs related to antimicrobials not structurally related to the AGP. AGPs did not impact the gut bacterial community structure, including alpha or beta diversity significantly, with only 16–20 operational taxonomic units (OTUs) of bacteria being altered significantly. However, several AGPs significantly reduced the population density of some of the potential pathogenic genera of bacteria, such as Escherichia coli. Chlortetracycline increased the abundance of Escherichia phage, whereas other AGPs did not influence the abundance of bacteriophage significantly. Considering the evidence that AGPs used in poultry production can select for resistance to more than one class of antimicrobial resistance, and the fact that their effect on performance is not significant, their use needs to be reduced and there is a need to monitor the spread of ARGs in broiler chicken farms.
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影响因子：
4.4
作者：
Maria Cardinal K;Kipper M;Andretta I;Machado Leal Ribeiro A
通讯作者：
Machado Leal Ribeiro A
影响因子：
4.8
作者：
Glassman SI;Martiny JBH
通讯作者：
Martiny JBH
影响因子：
14.9
作者：
Arndt D;Xia J;Liu Y;Zhou Y;Guo AC;Cruz JA;Sinelnikov I;Budwill K;Nesbø CL;Wishart DS
通讯作者：
Wishart DS
DOI：
10.1093/jac/dkr311
发表时间：
2011-10
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
Ciric L;Mullany P;Roberts AP
通讯作者：
Roberts AP
影响因子：
3.7
作者：
Costa MC;Bessegatto JA;Alfieri AA;Weese JS;Filho JA;Oba A
通讯作者：
Oba A