Antibiotic and antiseptic resistance genes are linked on a novel mobile genetic element: Tn6087.
Antibiotic and antiseptic resistance genes are linked on a novel mobile genetic element: Tn6087.
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DOI：
10.1093/jac/dkr311
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Roberts AP
中科院分区：
文献类型：
--
作者：
Ciric L;Mullany P;Roberts AP
Tn 916-like元件是整合接合元件（ICE）中最常见的类型之一。在这项研究中，我们的目的是确定是否新的辅助基因，即基因的产品不参与流动性或调节，是目前的Tn 916样元件（Tn 6087）从口腔链球菌从人类口腔分离。米诺环素耐药分离株进行了分析，使用限制性片段长度多态性（RFLP）分析的扩增子来自Tn 916和DNA测序，以确定是否有遗传差异Tn 6087相比，Tn 916。突变分析被用来确定是否发现的新的辅助基因是负责观察到的额外的表型。描述了一种新的Tn 916样元件Tn 6087，其编码抗生素和防腐剂抗性。抗菌剂抗性蛋白由一种新的小的多药耐药基因编码，命名为qrg，该基因被证明编码对十六烷基三甲基溴化铵（CTAB）的抗性，也称为溴化西曲溴铵。这是第一个描述的赋予抗生素和防腐剂抗性的Tn 916样元件，表明抗生素或防腐剂抗性的选择也将选择另一种，并进一步强调了谨慎使用这两种类型化合物的必要性。
Tn916-like elements are one of the most common types of integrative and conjugative element (ICE). In this study we aimed to determine whether novel accessory genes, i.e. genes whose products are not involved in mobility or regulation, were present on a Tn916-like element (Tn6087) isolated from Streptococcus oralis from the human oral cavity. A minocycline-resistant isolate was analysed using restriction fragment length polymorphism (RFLP) analysis on amplicons derived from Tn916 and DNA sequencing to determine whether there were genetic differences in Tn6087 compared with Tn916. Mutational analysis was used to determine whether the novel accessory gene found was responsible for an observed extra phenotype. A novel Tn916-like element, Tn6087, is described that encodes both antibiotic and antiseptic resistance. The antiseptic resistance protein is encoded by a novel small multidrug resistance gene, designated qrg, that was shown to encode resistance to cetyltrimethylammonium bromide (CTAB), also known as cetrimide bromide. This is the first Tn916-like element described that confers both antibiotic and antiseptic resistance, suggesting that selection of either antibiotic or antiseptic resistance will also select for the other and further highlights the need for prudent use of both types of compound.
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影响因子：
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