Biomechanical effects of flow and coculture on human aortic and cord blood-derived endothelial cells.
Biomechanical effects of flow and coculture on human aortic and cord blood-derived endothelial cells.
复制标题
流量和共培养对人主动脉和脐带血源性内皮细胞的生物力学作用。
DOI：
10.1016/j.jbiomech.2011.05.024
复制
发表时间：
2011-07-28
影响因子：
2.4
通讯作者：
Truskey, George A.
中科院分区：
文献类型：
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作者：
Cao, Li;Wu, Andrew;Truskey, George A.
关键词：
人脐带血内皮细胞（hCB-ECs）是组织工程血管内皮化的一个很有前途的细胞来源。直接在模拟天然和组织工程血管的人主动脉平滑肌细胞（SMC）上培养的hCB-EC产生汇合的内皮，其像正常人主动脉内皮细胞（HAEC）一样响应流动。本研究的目的是定量的弹性模量的hCB-ECs与SMC在静态和流动条件下，使用原子力显微镜（AFM）。通过AFM细胞表面成像和F-actin的免疫荧光来评估细胞骨架结构。静态条件下，hCB-ECs和HAECs的弹性模量相似，且显着小于单一培养SMC的弹性模量值（p<0.05）。在共培养中，hCB-ECs和HAECs变得显著更硬，模量比单一培养中的相应值大160 - 180%。而hCB-ECs和HAECs的模量几乎翻了一番，在单一培养和流动条件下，其相应的值在共培养后，暴露于流动下降。在共培养和/或流动条件下，每细胞宽度的皮质应力纤维数量和直径均增加，而整个细胞内部的皮质下应力纤维密度增加较少。这些研究结果表明，在共培养和/或暴露于流的生物力学特性的变化与皮质应力纤维密度的变化。对于内皮细胞，流体剪切应力似乎有更大的影响，弹性模量比SMC的存在和变化的弹性模量在共培养可能是由于EC-SMC通信。
Human endothelial cells derived from umbilical cord blood (hCB-ECs) represent a promising cell source for endothelialization of tissue engineered blood vessels. hCB-ECs cultured directly above human aortic smooth muscle cells (SMCs), which models native and tissue engineered blood vessels, produce a confluent endothelium that responds to flow like normal human aortic endothelial cells (HAECs). The objective of this study was to quantify the elastic modulus of hCB-ECs cocultured with SMCs under static and flow conditions using atomic force microscopy (AFM). Cytoskeleton structures were assessed by AFM cell surface imaging and immunofluorescence of F-actin. The elastic moduli of hCB-ECs and HAECs were similar and significantly smaller than the value for SMCs in monoculture under static conditions (p<0.05). In coculture, hCB-ECs and HAECs became significantly stiffer with moduli 160 – 180% larger than their corresponding values in monoculture. While the moduli of hCB-ECs and HAECs almost doubled in monoculture and flow condition, their corresponding values in coculture declined after exposure to flow. Both the number and diameter of cortical stress fiber per cell width increased in coculture and/or flow conditions, whereas the subcortical stress fiber density throughout the cell interior increased by a smaller amount. These findings indicate that changes to biomechanical properties in coculture and/or exposure to flow are correlated with changes in the cortical stress fiber density. For ECs, fluid shear stress appeared to have greater effect on the elastic modulus than the presence of SMCs and changes to the elastic modulus in coculture may be due to EC-SMC communication.
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影响因子：
64.8
作者：
FRANKE, RP;GRAFE, M;DRENCKHAHN, D
通讯作者：
DRENCKHAHN, D
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Truskey, GA
影响因子：
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作者：
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通讯作者：
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影响因子：
64.8
作者：
通讯作者：
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