Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells.
Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells.
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DOI：
10.1038/s41598-017-05207-1
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发表时间：
2017-07-11
影响因子：
4.6
通讯作者：
Wagner W
中科院分区：
文献类型：
--
作者：
Fernandez-Rebollo E;Mentrup B;Ebert R;Franzen J;Abagnale G;Sieben T;Ostrowska A;Hoffmann P;Roux PF;Rath B;Goodhardt M;Lemaitre JM;Bischof O;Jakob F;Wagner W
间充质基质细胞（MSC）的培养基通常补充有人血小板裂解物（HPL）或胎牛血清（FCS）。许多研究表明，增殖和细胞形态受到这些补充剂的影响-因此，重要的是要确定它们是否有利于不同亚群的生长，从而影响MSC的异质性组成。我们已经分离并扩增了人骨髓来源的MSC与HPL或FCS平行，并证明HPL显著增加增殖并导致细胞形态的显著差异。值得注意的是，全球DNA甲基化图谱没有显示任何显著差异。即使在转录组水平，在成对比较中也只有中度变化。此外，对增殖，细胞骨架组织，和局灶性粘连的影响是可逆的互换到相反的培养条件。这些结果表明，用HPL或FCS培养MSC对特定细胞类型没有系统偏倚。
Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements – it is therefore important to determine if they favor outgrowth of different subpopulations and thereby impact on the heterogeneous composition of MSCs. We have isolated and expanded human bone marrow-derived MSCs in parallel with HPL or FCS and demonstrated that HPL significantly increases proliferation and leads to dramatic differences in cellular morphology. Remarkably, global DNA-methylation profiles did not reveal any significant differences. Even at the transcriptomic level, there were only moderate changes in pairwise comparison. Furthermore, the effects on proliferation, cytoskeletal organization, and focal adhesions were reversible by interchanging to opposite culture conditions. These results indicate that cultivation of MSCs with HPL or FCS has no systematic bias for specific cell types.
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影响因子：
5.6
作者：
Doucet, C;Ernou, I;Lataillade, JJ
通讯作者：
Lataillade, JJ
影响因子：
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Abagnale, Giulio;Steger, Michael;Wagner, Wolfgang
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影响因子：
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Strunk, Dirk
影响因子：
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作者：
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