A STE12 homolog is required for mating but dispensable for filamentation in candida lusitaniae.
A STE12 homolog is required for mating but dispensable for filamentation in candida lusitaniae.
复制标题
在卢西塔尼亚假丝酵母中，交配需要 STE12 同系物，但丝状形成则不需要。
DOI：
10.1093/genetics/155.1.17
复制
发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Heitman,J
中科院分区：
文献类型：
--
作者：
Young,LY;Lorenz,MC;Heitman,J
Candida lusitaniaeis a dimorphic yeast that is emerging as an opportunistic fungal pathogen. In contrast toCandida albicans, which is diploid and asexual,C. lusitaniaehas been reported to have a sexual cycle. We have employed genetic approaches to demonstrate thatC. lusitaniaeis haploid and has a sexual cycle involving mating betweenMATaandMATα cells under nutrient deprivation conditions. By degenerate PCR, we identified aC. lusitaniaehomolog (Cls12) of the Ste12 transcription factor that regulates mating, filamentation, and virulence inSaccharomyces cerevisiae, C. albicans, andCryptococcus neoformans.Comparison of theCLS12DNA and protein sequences to otherSTE12homologs and transformation experiments with selectable markers fromS. cerevisiae(URA3, KanMX, HphMX) andC. albicans(CaURA3) provide evidence that the CUG codon encodes serine instead of leucine inC. lusitaniae, as is also the case inC. albicans.TheC. lusitaniae CLS12gene was disrupted by biolistic transformation and homologous recombination.C. lusitaniae cls12mutant strains were sterile but had no defect in filamentous growth. Our findings reveal both conserved and divergent roles for theC. lusitaniae STE12homolog in regulating differentiation of this emerging fungal pathogen.
登录
查看更多内容
影响因子：
3.6
作者：
A. Léan;A. Hancock;R. Lefkowitz
通讯作者：
A. Léan;A. Hancock;R. Lefkowitz
影响因子：
64.5
作者：
MIYAJIMA, I;NAKAFUKU, M;MATSUMOTO, K
通讯作者：
MATSUMOTO, K
影响因子：
64.5
作者：
DIETZEL, C;KURJAN, J
通讯作者：
KURJAN, J
影响因子：
64.8
作者：
CHEE, MS;SATCHWELL, SC;BARRELL, BG
通讯作者：
BARRELL, BG
使用 ADCI 启动子在酵母中表达基因。
影响因子：
--
作者：
G. Ammerer
通讯作者：
G. Ammerer