A new approach to quantify system efficiency with dissolved oxygen isotopes during engineered growth of Galdieria sulphuraria
A new approach to quantify system efficiency with dissolved oxygen isotopes during engineered growth of Galdieria sulphuraria
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在 Galdieria sulfuraria 工程化生长过程中用溶解氧同位素量化系统效率的新方法
DOI：
10.1016/j.algal.2017.07.026
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发表时间：
2017
影响因子：
5.1
通讯作者：
J.A.C.
中科院分区：
文献类型：
--
作者：
Schwerna;Buchholz;van Geldern;J.A.C.
微藻Galdieria sulphuraria以第一技术方法在封闭系统实验中生长29天。在此时间段内，对6个单独的烧瓶进行取样，以测定溶解相和顶空相中的氧浓度及其稳定同位素比（18 O/16 O）。还分析了水的氧同位素组成，作为通过藻类生产将其18 O/16 O比率转移为分子氧的输入。这种光合作用转移的同位素组成的水平衡的氧消耗，丰富的18 O两个阶段。因此，尽管光合作用过度，但溶解氧和顶部空间中的氧都没有达到H2O的18 O-贫化氧同位素比。光合作用产生的氧气在顶空中积累了13.96 mmol L− 1，在液相中积累了0.5 mmol L− 1。这种差异是由于溶液快速脱气所致。它被进一步放大的溶解O2相的优先消耗。为了量化氧的产生和消耗，我们确定了光合作用/呼吸（P/R）的比率与公式相结合的O2浓度和其同位素比率。11天后，P/R比为7.7。此后，它又下降，并走向呼吸优势。通过这项工作，我们的研究结果介绍了一种新的方法来监测藻类的生长和效率的控制实验。
The microalgaeGaldieria sulphurariawas grown in a first technical approach in a closed system experiment for 29 days. In this time period, 6 separate flasks were sampled for oxygen concentration and their stable isotope ratios (18O/16O) in dissolved and headspace phases. The oxygen isotope composition of the water was also analysed as an input for the transfer of its18O/16O ratio to molecular oxygen via production by algae. This photosynthetic transfer of the isotope composition of water was counterbalanced by oxygen consumption that enriched both phases in18O. For this reason, neither dissolved oxygen nor oxygen in the headspace reached the18O-depleted oxygen isotope ratio of H2O despite excessive photosynthesis. Oxygen that was produced by photosynthesis accumulated with a yield of 13.96 mmol L− 1in the headspace and with 0.5 mmol L− 1in the fluid phase. This difference was due to rapid degassing of the solution. It was further amplified by preferential consumption of the dissolved O2phase. In order to quantify oxygen production and its consumption we determined photosynthesis/respiration (P/R) ratios with a formula that combined O2concentrations and its isotope ratios. It revealed a P/R ratio of 7.7 after 11 days. After this it decreased again and moved towards dominance of respiration. With this work our results introduce a new method to monitor the growth and efficiency of algae in controlled experiments.
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DOI：
10.1080/03067318908026878
发表时间：
1989-01-01
影响因子：
2.6
作者：
KAMPBELL, DH;WILSON, JT;VANDEGRIFT, SA
通讯作者：
VANDEGRIFT, SA
影响因子：
56.9
作者：
Schoenknecht, Gerald;Chen, Wei-Hua;Weber, Andreas P. M.
通讯作者：
Weber, Andreas P. M.
海洋表面氧同位素分馏和大气 O2 的 18O/16O
影响因子：
5.2
作者：
B. Luz;E. Barkan
通讯作者：
E. Barkan
影响因子：
4.1
作者：
Kuhne, S.;Strieth, D.;Ulber, R.
通讯作者：
Ulber, R.
气态氧和溶解氧的动力学和稳定同位素组成
影响因子：
2.6
作者：
L. Wassenaar;M. Hendry
通讯作者：
M. Hendry