Towards enhancing coral heat tolerance: a "microbiome transplantation" treatment using inoculations of homogenized coral tissues.
Towards enhancing coral heat tolerance: a "microbiome transplantation" treatment using inoculations of homogenized coral tissues.
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DOI：
10.1186/s40168-021-01053-6
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发表时间：
2021-05-06
期刊：
影响因子：
15.5
通讯作者：
Roik A
中科院分区：
文献类型：
--
作者：
Doering T;Wall M;Putchim L;Rattanawongwan T;Schroeder R;Hentschel U;Roik A
微生物组操纵可以提高耐热性，帮助珊瑚在海洋变暖的压力下生存。我们使用造礁珊瑚，Pocillopora和Porites进行了珊瑚微生物组移植（CMT）实验，并研究了这种技术是否可以在修改细菌微生物组的同时有益于珊瑚的耐热性。最初，耐热供体是在野外鉴定的。然后，我们使用新鲜的匀浆从珊瑚供体组织到同种的，热敏感的受体，并记录他们的漂白反应和微生物组的16 S rRNA基因metabarcoding。当暴露于短期热应激（34 °C）时，两种珊瑚物种的接受者与对照组相比以较低的速率漂白。112（Pocillopora sp.）和16个（Porites sp.）在接受者的微生物组中鉴定出供体特异性细菌物种，表明细菌的传播。大多数这些传播的细菌的扩增子序列变异属于已知的，pupelium共生细菌分类群的珊瑚，并与观察到的有益影响珊瑚应力响应。我们实验中的微生物组动态支持这样的观点：微生物组群落的均匀性和一种或几种细菌物种的优势，而不是宿主物种的身份，是全生物环境中微生物组稳定性的驱动因素。我们的研究结果表明，珊瑚受体可能有利于吸收假定的细菌共生体，建议将这些分类群体包括在未来的珊瑚益生菌筛选工作。我们的研究表明，与对照组中存在的原生共生体相比，这些供体特异性细菌共生体可能更有效地支持受体抵抗热应激。这些发现迫切需要进一步的实验研究的作用机制的基础上的有益效果的CMT和实地为基础的长期研究测试的持久性的效果。视频摘要在线版本包含补充材料，可在10. 1186/s40168-021-01053-6。
Microbiome manipulation could enhance heat tolerance and help corals survive the pressures of ocean warming. We conducted coral microbiome transplantation (CMT) experiments using the reef-building corals, Pocillopora and Porites, and investigated whether this technique can benefit coral heat resistance while modifying the bacterial microbiome. Initially, heat-tolerant donors were identified in the wild. We then used fresh homogenates made from coral donor tissues to inoculate conspecific, heat-susceptible recipients and documented their bleaching responses and microbiomes by 16S rRNA gene metabarcoding. Recipients of both coral species bleached at lower rates compared to the control group when exposed to short-term heat stress (34 °C). One hundred twelve (Pocillopora sp.) and sixteen (Porites sp.) donor-specific bacterial species were identified in the microbiomes of recipients indicating transmission of bacteria. The amplicon sequence variants of the majority of these transmitted bacteria belonged to known, putatively symbiotic bacterial taxa of corals and were linked to the observed beneficial effect on the coral stress response. Microbiome dynamics in our experiments support the notion that microbiome community evenness and dominance of one or few bacterial species, rather than host-species identity, were drivers for microbiome stability in a holobiont context. Our results suggest that coral recipients likely favor the uptake of putative bacterial symbionts, recommending to include these taxonomic groups in future coral probiotics screening efforts. Our study suggests a scenario where these donor-specific bacterial symbionts might have been more efficient in supporting the recipients to resist heat stress compared to the native symbionts present in the control group. These findings urgently call for further experimental investigation of the mechanisms of action underlying the beneficial effect of CMT and for field-based long-term studies testing the persistence of the effect. Video abstract The online version contains supplementary material available at 10.1186/s40168-021-01053-6.
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影响因子：
9.8
作者：
Bojanova DP;Bordenstein SR
通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/j.jembe.2015.06.014
发表时间：
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影响因子：
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