Microfluidic compartmentalized co-culture platform for CNS axon myelination research.
Microfluidic compartmentalized co-culture platform for CNS axon myelination research.
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DOI：
10.1007/s10544-009-9331-7
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发表时间：
2009-12
影响因子：
2.8
通讯作者：
Han, Arum
中科院分区：
文献类型：
--
作者：
Park, Jaewon;Koito, Hisami;Li, Jianrong;Han, Arum
本文提出了一种圆形微流体区室化共培养平台，可用于中枢神经系统（CNS）轴突髓鞘化研究。微流体平台由体细胞室和轴突/神经胶质室组成，通过轴突引导微通道阵列连接。产生髓磷脂的神经胶质细胞，少突胶质细胞（OL），放置在轴突/神经胶质细胞中，仅与轴突相互作用，但不与局限于体细胞室的神经元体细胞相互作用，这让人想起体内的情况，其中许多轴突纤维在远离神经元细胞体的地方被OLs有髓鞘化。来自胚胎第 16-18 天的大鼠的初级前脑神经元在体细胞室内培养两周，以使它们成熟并形成广泛的轴突网络。从出生后 1-2 天的大鼠大脑中分离出 OL 祖细胞，然后将其添加到轴突/神经胶质室中，并与神经元共培养另外两周。该微型装置显示出两个区室之间的流体隔离，并成功地从轴突中分离出神经元细胞体和树突，这些轴突通过轴突引导微通道阵列生长到轴突/神经胶质区室中。这里开发的圆形共培养装置显示出优异的细胞装载特性，其中大量细胞位于轴突引导微通道附近。这显着增加了轴突穿过这些微通道的概率，细胞接种两周后超过 51% 的轴突/神经胶质区室面积被轴突覆盖就证明了这一点。 OL 祖细胞与轴突/神经胶质细胞内的轴突共培养，成功分化为成熟的 OL。这些结果表明该装置可用作研究局部轴突-神经胶质细胞相互作用和信号传导的优秀体外共培养平台。
This paper presents a circular microfluidic compartmentalized co-culture platform that can be used for central nervous system (CNS) axon myelination research. The microfluidic platform is composed of a soma compartment and an axon/glia compartment connected through arrays of axon-guiding microchannels. Myelin-producing glia, oligodendrocytes (OLs), placed in the axon/glia compartment, interact with only axons but not with neuronal somata confined to the soma compartment, reminiscent to in vivo situation where many axon fibres are myelinated by OLs at distance away from neuronal cell bodies. Primary forebrain neurons from embryonic day 16–18 rats were cultured inside the soma compartment for two weeks to allow them to mature and form extensive axon networks. OL progenitors, isolated from postnatal day 1–2 rat brains, were then added to the axon/glia compartment and co-cultured with neurons for an additional two weeks. The microdevice showed fluidic isolation between the two compartments and successfully isolated neuronal cell bodies and dendrites from axons growing through the arrays of axon-guiding microchannels into the axon/glia compartment. The circular co-culture device developed here showed excellent cell loading characteristics where significant numbers of cells were positioned near the axon-guiding microchannels. This significantly increased the probability of axons crossing these microchannels as demonstrated by the more than 51% of the area of the axon/glia compartment covered with axons two weeks after cell seeding. OL progenitors co-cultured with axons inside the axon/glia compartment successfully differentiated into mature OLs. These results indicate that this device can be used as an excellent in vitro co-culture platform for studying localized axon-glia interaction and signalling.
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DOI：
10.1073/pnas.0502552102
发表时间：
2005-07-12
影响因子：
11.1
作者：
Li, JR;Baud, O;Rosenberg, PA
通讯作者：
Rosenberg, PA
影响因子：
6.1
作者：
Millet, Larry J.;Stewart, Matthew E.;Gillette, Martha U.
通讯作者：
Gillette, Martha U.
影响因子：
3
作者：
Chao, ZC;Bakkum, DJ;Potter, SM
通讯作者：
Potter, SM
影响因子：
12.6
作者：
Thiébaud, P;Lauer, L;Offenhäusser, A
通讯作者：
Offenhäusser, A
DOI：
10.1073/pnas.0607394104
发表时间：
2006-12-26
影响因子：
11.1
作者：
Bi, Jing;Tsai, Nien-Pei;Wei, Li-Na
通讯作者：
Wei, Li-Na