Heat-Induced Oxidation of the Nuclei and Cytosol.
Heat-Induced Oxidation of the Nuclei and Cytosol.
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DOI：
10.3389/fpls.2020.617779
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发表时间：
2020
影响因子：
5.6
通讯作者：
Foyer CH
中科院分区：
文献类型：
--
作者：
Babbar R;Karpinska B;Grover A;Foyer CH
人们普遍认为，热应激(HS)会导致活性氧物种(ROS)的大量积累。然而，ROS积累的细胞内分区一直没有得到很好的描述。因此，我们使用氧化还原敏感的绿色荧光蛋白(RoGFP2)来提供关于热诱导拟南芥叶片、表皮和气孔保卫细胞的细胞核和胞浆的氧化还原变化的区室特异性信息。我们发现，HS导致两个隔室的氧化程度大幅增加，导致细胞的谷胱甘肽氧化还原电位发生大幅移动。热诱导的标记物转录本、热休克蛋白(HSP)101和抗坏血酸过氧化物酶(APX)2的水平在热处理开始后15min达到最大。对对照和热处理幼苗的转录谱进行的RNAseq分析表明，编码HSPs、线粒体蛋白、转录因子和其他核定位成分的转录产物发生了巨大变化。我们的结论是，HS导致细胞核和胞浆的广泛氧化。我们认为，热诱导的核氧化还原状态的变化是植物对HS反应的遗传和表观遗传控制的中心。
The concept that heat stress (HS) causes a large accumulation of reactive oxygen species (ROS) is widely accepted. However, the intracellular compartmentation of ROS accumulation has been poorly characterized. We therefore used redox-sensitive green fluorescent protein (roGFP2) to provide compartment-specific information on heat-induced redox changes of the nuclei and cytosol of Arabidopsis leaf epidermal and stomatal guard cells. We show that HS causes a large increase in the degree of oxidation of both compartments, causing large shifts in the glutathione redox potentials of the cells. Heat-induced increases in the levels of the marker transcripts, heat shock protein (HSP)101, and ascorbate peroxidase (APX)2 were maximal after 15 min of the onset of the heat treatment. RNAseq analysis of the transcript profiles of the control and heat-treated seedlings revealed large changes in transcripts encoding HSPs, mitochondrial proteins, transcription factors, and other nuclear localized components. We conclude that HS causes extensive oxidation of the nucleus as well as the cytosol. We propose that the heat-induced changes in the nuclear redox state are central to both genetic and epigenetic control of plant responses to HS.
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影响因子：
7.3
作者：
Groten, K;Vanacker, H;Foyer, CH
通讯作者：
Foyer, CH
影响因子：
8.8
作者：
Dickinson PJ;Kumar M;Martinho C;Yoo SJ;Lan H;Artavanis G;Charoensawan V;Schöttler MA;Bock R;Jaeger KE;Wigge PA
通讯作者：
Wigge PA
影响因子：
5.6
作者：
Driedonks N;Xu J;Peters JL;Park S;Rieu I
通讯作者：
Rieu I
影响因子：
4.8
作者：
Hanson, GT;Aggeler, R;Remington, SJ
通讯作者：
Remington, SJ
影响因子：
6.6
作者：
de Simone A;Hubbard R;de la Torre NV;Velappan Y;Wilson M;Considine MJ;Soppe WJJ;Foyer CH
通讯作者：
Foyer CH