Redox Changes During the Cell Cycle in the Embryonic Root Meristem of Arabidopsis thaliana.
Redox Changes During the Cell Cycle in the Embryonic Root Meristem of Arabidopsis thaliana.
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DOI：
10.1089/ars.2016.6959
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发表时间：
2017-12-20
影响因子：
6.6
通讯作者：
Foyer CH
中科院分区：
文献类型：
--
作者：
de Simone A;Hubbard R;de la Torre NV;Velappan Y;Wilson M;Considine MJ;Soppe WJJ;Foyer CH
目的：本研究的目的是表征发芽拟南芥幼苗胚胎根有丝分裂细胞周期期间细胞核和细胞质中发生的氧化还原变化，并确定抗坏血酸合成能力下降的突变体中氧化还原循环是如何改变的。结果：使用体内还原氧化（氧化还原）报告基因（roGFP2），我们发现拟南芥根顶端分生组织的同步分裂细胞在 G1 时发生细胞质和细胞核的瞬时氧化，在 G2 和有丝分裂时发生还原。低抗坏血酸突变体不存在这种氧化还原循环，其中细胞核比对照明显氧化更多。在抗坏血酸缺陷突变体中，细胞周期依赖性的核大小增加受到损害，其根增殖区每单位面积的细胞较少。干燥种子的转录谱和吸湿种子的大小受到低抗坏血酸的强烈影响，但发芽、休眠释放和种子老化特性不受影响。创新：这些数据证明植物细胞周期中存在氧化还原循环，并且细胞核的氧化还原状态是细胞周期进程的重要因素。结论：受控氧化是植物细胞周期早期的一个关键特征。然而，持续的轻度氧化会限制核功能并损害细胞周期的进展，导致根尖分生组织中的细胞减少。抗氧化剂。氧化还原信号。 27, 1505–1519。
Aims: The aim of this study was to characterize redox changes in the nuclei and cytosol occurring during the mitotic cell cycle in the embryonic roots of germinating Arabidopsis seedlings, and to determine how redox cycling was modified in mutants with a decreased capacity for ascorbate synthesis. Results: Using an in vivo reduction-oxidation (redox) reporter (roGFP2), we show that transient oxidation of the cytosol and the nuclei occurred at G1 in the synchronized dividing cells of the Arabidopsis root apical meristem, with reduction at G2 and mitosis. This redox cycle was absent from low ascorbate mutants in which nuclei were significantly more oxidized than controls. The cell cycle-dependent increase in nuclear size was impaired in the ascorbate-deficient mutants, which had fewer cells per unit area in the root proliferation zone. The transcript profile of the dry seeds and size of the imbibed seeds was strongly influenced by low ascorbate but germination, dormancy release and seed aging characteristics were unaffected. Innovation: These data demonstrate the presence of a redox cycle within the plant cell cycle and that the redox state of the nuclei is an important factor in cell cycle progression. Conclusions: Controlled oxidation is a key feature of the early stages of the plant cell cycle. However, sustained mild oxidation restricts nuclear functions and impairs progression through the cell cycle leading to fewer cells in the root apical meristem. Antioxid. Redox Signal. 27, 1505–1519.
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影响因子：
5.6
作者：
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