Unveiling Mycoplasma hyopneumoniae promoters: sequence definition and genomic distribution.
Unveiling Mycoplasma hyopneumoniae promoters: sequence definition and genomic distribution.
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DOI：
10.1093/dnares/dsr045
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发表时间：
2012-04
期刊：
影响因子：
--
通讯作者：
Schrank IS
中科院分区：
文献类型：
--
作者：
Weber Sde S;Sant'Anna FH;Schrank IS
几种支原体的基因组已经完全测序，包括四种猪病原体猪支原体。然而，很少有人知道的核苷酸序列，控制转录起始在这些微生物。因此，为了研究M.通过对不同基因的23个转录起始位点（TSS）的定位，初步确定了不同基因的转录起始位点（TSS）。在TSS的上游发现了类似于σ70启动子-10元件的模式。然而，没有-35元素被区分。相反，AT丰富的周期性信号被识别。大约一半的实验定义的启动子含有基序5′-TRTGn-3′，这与革兰氏阳性细菌中常见的−16元件相同。利用确定的启动子建立位置特异性评分矩阵，以扫描M.猪舌草基因组201个信号与169个CDS相关。这些序列中的大多数位于起始密码子的100个核苷酸内。这项研究表明，启动子样序列的数量在M。然而，在猪流感基因组中，偶然发现的序列比预期的更频繁，表明检测到的大多数序列可能具有生物学功能。
Several Mycoplasma species have had their genome completely sequenced, including four strains of the swine pathogen Mycoplasma hyopneumoniae. Nevertheless, little is known about the nucleotide sequences that control transcriptional initiation in these microorganisms. Therefore, with the objective of investigating the promoter sequences of M. hyopneumoniae, 23 transcriptional start sites (TSSs) of distinct genes were mapped. A pattern that resembles the σ70 promoter −10 element was found upstream of the TSSs. However, no −35 element was distinguished. Instead, an AT-rich periodic signal was identified. About half of the experimentally defined promoters contained the motif 5′-TRTGn-3′, which was identical to the −16 element usually found in Gram-positive bacteria. The defined promoters were utilized to build position-specific scoring matrices in order to scan putative promoters upstream of all coding sequences (CDSs) in the M. hyopneumoniae genome. Two hundred and one signals were found associated with 169 CDSs. Most of these sequences were located within 100 nucleotides of the start codons. This study has shown that the number of promoter-like sequences in the M. hyopneumoniae genome is more frequent than expected by chance, indicating that most of the sequences detected are probably biologically functional.
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