Pulmonary vascular protein sieving capability after exposure to high vascular pressures.
Pulmonary vascular protein sieving capability after exposure to high vascular pressures.
复制标题
暴露于高血管压力后肺血管蛋白筛分能力。
DOI：
10.1152/jappl.1992.73.1.50
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Jarjoura,DG
中科院分区：
文献类型：
--
作者：
Bosso,FJ;Maron,MB;Pilati,CF;Jarjoura,DG
在恒流条件下，将犬左下叶动脉压短暂升高至23.8+/-0.9(对照组，n=5)或92.3+/-1.4Torr(高压组，n=9)后，观察犬原位左下叶血管对不同分子半径(34-124A)内源性血浆蛋白的筛选能力。在微循环血流恢复自然灌流后，逐步增加左房压力，采集微循环淋巴和血液样本，直到获得非滤过性淋巴与血浆蛋白浓度比(CL/CP)。计算了总蛋白质和7种蛋白质组分(梯度凝胶电泳法)的渗透反射系数(Sigma D)。高压组总蛋白平均值[0.51+/-0.06(SE)]显著低于对照组(0.68+/-0.03)。然而，高压组的几个LLL显示正常的Sigma‘s，两组的蛋白质组分CL/Cp’s随分子半径的增大而降低，但高压组的CL/Cp-分子半径关系上移。孔隙分析表明，Sigma d的降低可以用通过大孔隙系统的分数流量的增加来解释。
We evaluated the ability of the canine in situ left lower lobe (LLL) vasculature to sieve endogenous plasma proteins of various molecular radii (34–124 A) after LLL arterial pressure had been transiently elevated to 23.8 +/- 0.9 (control group, n = 5) or 92.3 +/- 1.4 (SE) Torr (high-pressure group, n = 9) by restricting LLL venous outflow under conditions of constant flow. After LLL flow was returned to natural perfusion, left atrial pressure was elevated in step increments, and LLL lymph and blood samples were collected until filtration-independent lymph-to-plasma protein concentration ratios (CL/CP) were obtained. The osmotic reflection coefficients (sigma d) for total proteins and seven protein fractions (separated by gradient gel electrophoresis) were calculated. The average total protein sigma d of the high-pressure group [0.51 +/- 0.06 (SE)] was significantly lower than that of the control group (0.68 +/- 0.03). Several LLLs of the high-pressure group, however, exhibited normal sigma d′s. Protein fraction CL/CP′s decreased with increasing molecular radius in both groups, but the CL/CP-molecular radius relationship was displaced upward in the high-pressure group. Pore analysis suggested that the decreases in sigma d could be explained by increases in the fractional flow through a large-pore system.
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