Antimony efflux underpins phosphorus cycling and resistance of phosphate-solubilizing bacteria in mining soils.
Antimony efflux underpins phosphorus cycling and resistance of phosphate-solubilizing bacteria in mining soils.
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DOI：
10.1038/s41396-023-01445-6
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发表时间：
2023-08
期刊：
影响因子：
11
通讯作者：
Yan, Qingyun
中科院分区：
文献类型：
--
作者：
Liu, Shengwei;Zeng, Jiaxiong;Yu, Huang;Wang, Cheng;Yang, Yunfeng;Wang, Jianjun;He, Zhili;Yan, Qingyun
微生物在重金属污染土壤中磷的周转和磷的生物有效性增加中起着至关重要的作用。然而，微生物驱动的磷循环过程和机制，他们对重金属污染物的抗性仍然知之甚少。在这里，我们研究了可能的生存策略的P-循环微生物在水平和垂直土壤样品从世界上最大的锑（Sb）采矿场，这是位于锡矿山，中国。结果表明，土壤总锑和pH是影响土壤细菌群落多样性、结构和磷循环特性的主要因素。细菌与gcd基因，编码的酶负责葡萄糖酸的生产，在很大程度上与无机磷（Pi）的溶解和显着提高土壤磷的生物有效性。在回收的106个几乎完整的细菌宏基因组组装基因组（MAG）中，60.4%携带gcd基因。pit或pstSCAB编码的Pi转运系统广泛存在于gcd携带细菌中，并且43.8%的gcd携带细菌还携带编码Sb外排泵的acr 3基因。系统发育和潜在的水平基因转移（HGT）的acr 3的分析表明，锑流出可能是一个显性的抗性机制，和两个gcd窝藏MAG出现获得acr 3通过HGT。结果表明，锑的外排可增强矿区土壤中磷的循环和溶磷细菌对重金属的抗性。该研究为重金属污染生态系统的治理和修复提供了新的策略。
Microorganisms play crucial roles in phosphorus (P) turnover and P bioavailability increases in heavy metal-contaminated soils. However, microbially driven P-cycling processes and mechanisms of their resistance to heavy metal contaminants remain poorly understood. Here, we examined the possible survival strategies of P-cycling microorganisms in horizontal and vertical soil samples from the world’s largest antimony (Sb) mining site, which is located in Xikuangshan, China. We found that total soil Sb and pH were the primary factors affecting bacterial community diversity, structure and P-cycling traits. Bacteria with the gcd gene, encoding an enzyme responsible for gluconic acid production, largely correlated with inorganic phosphate (Pi) solubilization and significantly enhanced soil P bioavailability. Among the 106 nearly complete bacterial metagenome-assembled genomes (MAGs) recovered, 60.4% carried the gcd gene. Pi transportation systems encoded by pit or pstSCAB were widely present in gcd-harboring bacteria, and 43.8% of the gcd-harboring bacteria also carried the acr3 gene encoding an Sb efflux pump. Phylogenetic and potential horizontal gene transfer (HGT) analyses of acr3 indicated that Sb efflux could be a dominant resistance mechanism, and two gcd-harboring MAGs appeared to acquire acr3 through HGT. The results indicated that Sb efflux could enhance P cycling and heavy metal resistance in Pi-solubilizing bacteria in mining soils. This study provides novel strategies for managing and remediating heavy metal-contaminated ecosystems.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1073/pnas.2001063117
发表时间：
2020-05-12
影响因子：
11.1
作者：
Chen, Song-Can;Sun, Guo-Xin;Zhu, Yong-Guan
通讯作者：
Zhu, Yong-Guan
影响因子：
5.1
作者：
Bergkemper, Fabian;Schoeler, Anne;Schulz, Stefanie
通讯作者：
Schulz, Stefanie
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
关键真菌类群参与亚热带土壤有机磷矿化以及活性碳影响的证据
影响因子：
9.7
作者：
Chen, Yan;Sun, Ruibo;Sun, Bo
通讯作者：
Sun, Bo