Endolymphatic Potential Measured From Developing and Adult Mouse Inner Ear.
Endolymphatic Potential Measured From Developing and Adult Mouse Inner Ear.
复制标题
从发育中和成年小鼠内耳测量内淋巴电位
DOI：
10.3389/fncel.2020.584928
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发表时间：
2020
影响因子：
5.3
通讯作者：
He DZ
中科院分区：
文献类型：
--
作者：
Li Y;Liu H;Zhao X;He DZ
哺乳动物的内耳有两个主要部分，耳蜗负责听觉，前庭器官负责平衡。耳蜗和前庭器官通过颞骨中的一系列管道连接，并且两种不同的细胞外液，内淋巴和外淋巴，填充内耳的不同隔室。耳蜗和前庭终器中机械敏感毛细胞的静纤毛沐浴在内淋巴中，内淋巴含有高K+离子并具有称为内淋巴电位（ELP）的正电位。流体的区室化为毛细胞机械转导提供了电化学梯度。在这项研究中，我们测量ELP从成年和新生C57 BL/6 J小鼠，以确定如何ELP的变化和发展的耳蜗和前庭内淋巴。我们测量ELP和前庭颤噪反应从球囊的新生小鼠，以确定何时前庭功能成熟。我们发现，ELP在成年小鼠的耳蜗和前庭内淋巴中变化很大，范围从耳蜗的基底圈的+95 mV到顶端圈的+87 mV，球囊和椭圆囊的+9 mV，半规管的+3 mV。这表明ELP确实是一种局部潜力，尽管内淋巴组成相似。我们进一步表明，前庭ELP在出生后6天左右达到成人样大小，比耳蜗ELP的成熟早12天（即，耳蜗内电位）。前庭ELP的成熟与从囊状斑记录的前庭颤噪反应的成熟一致，表明小鼠前庭功能的成熟比听力的成熟早得多。
The mammalian inner ear has two major parts, the cochlea is responsible for hearing and the vestibular organ is responsible for balance. The cochlea and vestibular organs are connected by a series of canals in the temporal bone and two distinct extracellular fluids, endolymph and perilymph, fill different compartments of the inner ear. Stereocilia of mechanosensitive hair cells in the cochlea and vestibular end organs are bathed in the endolymph, which contains high K+ ions and possesses a positive potential termed endolymphatic potential (ELP). Compartmentalization of the fluids provides an electrochemical gradient for hair cell mechanotransduction. In this study, we measured ELP from adult and neonatal C57BL/6J mice to determine how ELP varies and develops in the cochlear and vestibular endolymph. We measured ELP and vestibular microphonic response from saccules of neonatal mice to determine when vestibular function is mature. We show that ELP varies considerably in the cochlear and vestibular endolymph of adult mice, ranging from +95 mV in the basal turn to +87 mV in the apical turn of the cochlea, +9 mV in the saccule and utricle, and +3 mV in the semicircular canal. This suggests that ELP is indeed a local potential, despite the fact that endolymph composition is similar. We further show that vestibular ELP reaches adult-like magnitude around post-natal day 6, ~12 days earlier than maturation of cochlear ELP (i.e., endocochlear potential). Maturation of vestibular ELP coincides with the maturation of vestibular microphonic response recorded from the saccular macula, suggesting that maturation of vestibular function occurs much earlier than maturation of hearing in mice.
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影响因子：
5.5
作者：
BOSHER, SK;WARREN, RL
通讯作者：
WARREN, RL
影响因子：
1.4
作者：
FERNANDEZ, C;HINOJOSA, R
通讯作者：
HINOJOSA, R
DOI：
10.1159/000275899
发表时间：
1987-01-01
期刊：
ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY AND ITS RELATED SPECIALTIES
影响因子：
--
作者：
NINOYU, O;HOMMERICH, C;MORGENSTERN, C
通讯作者：
MORGENSTERN, C
影响因子：
2.8
作者：
SADANAGA, M;MORIMITSU, T
通讯作者：
MORIMITSU, T
DOI：
10.1016/0010-406x(63)90105-1
发表时间：
1963-01-01
期刊：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY
影响因子：
--
作者：
SCHMIDT, RS
通讯作者：
SCHMIDT, RS