Priority effects dictate community structure and alter virulence of fungal-bacterial biofilms.
Priority effects dictate community structure and alter virulence of fungal-bacterial biofilms.
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DOI：
10.1038/s41396-021-00901-5
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Kalan LR
中科院分区：
文献类型：
--
作者：
Cheong JZA;Johnson CJ;Wan H;Liu A;Kernien JF;Gibson ALF;Nett JE;Kalan LR
多微生物生物膜是慢性伤口感染的标志。控制这些微生物生态系统的组装和成熟的力量在很大程度上尚未探索，但对宿主反应和临床结果的影响可能是显着的。在伤口愈合的情况下，生物膜和稳定的微生物群落结构的形成与受损的组织修复相关，导致慢性伤口不愈合。这些类型的伤口可以持续数年，低于经典定义的临床感染阈值（包括发热、疼痛、发红和肿胀），并在复发性感染阶段循环。在最严重的后果，截肢的下肢可能会发生，如果蔓延感染ensures。在这里，我们采取生态学的角度来研究优先级的影响和竞争性排斥的整体生物膜社区结构在一个三元社区组成的菌株的金黄色葡萄球菌，弗氏柠檬酸杆菌，白色念珠菌来自慢性伤口。我们发现，优先效应和细菌间的竞争结合C。白色念珠菌生物膜在非生物和生物基质（例如离体人皮肤伤口）上显著地形成群落结构。我们进一步证明了C. freundii到C.白念珠菌是由甘露糖结合凝集素介导的。C. freundii和C.白色念珠菌触发酵母菌到菌丝的转变，导致中性粒细胞死亡和炎症的显著增加。总的来说，这里提出的结果有助于我们理解真菌-细菌相互作用及其对宿主-微生物相互作用、发病机制和最终伤口愈合的影响。
Polymicrobial biofilms are a hallmark of chronic wound infection. The forces governing assembly and maturation of these microbial ecosystems are largely unexplored but the consequences on host response and clinical outcome can be significant. In the context of wound healing, formation of a biofilm and a stable microbial community structure is associated with impaired tissue repair resulting in a non-healing chronic wound. These types of wounds can persist for years simmering below the threshold of classically defined clinical infection (which includes heat, pain, redness, and swelling) and cycling through phases of recurrent infection. In the most severe outcome, amputation of lower extremities may occur if spreading infection ensues. Here we take an ecological perspective to study priority effects and competitive exclusion on overall biofilm community structure in a three-membered community comprised of strains of Staphylococcus aureus, Citrobacter freundii, and Candida albicans derived from a chronic wound. We show that both priority effects and inter-bacterial competition for binding to C. albicans biofilms significantly shape community structure on both abiotic and biotic substrates, such as ex vivo human skin wounds. We further show attachment of C. freundii to C. albicans is mediated by mannose-binding lectins. Co-cultures of C. freundii and C. albicans trigger the yeast-to-hyphae transition, resulting in a significant increase in neutrophil death and inflammation compared to either species alone. Collectively, the results presented here facilitate our understanding of fungal-bacterial interactions and their effects on host-microbe interactions, pathogenesis, and ultimately, wound healing.
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影响因子：
3.7
作者：
Dalton T;Dowd SE;Wolcott RD;Sun Y;Watters C;Griswold JA;Rumbaugh KP
通讯作者：
Rumbaugh KP
影响因子：
3.7
作者：
Dowd SE;Wolcott RD;Sun Y;McKeehan T;Smith E;Rhoads D
通讯作者：
Rhoads D
影响因子：
11.4
作者：
De Tender, Caroline;Devriese, Lisa I.;Ruttink, Tom
通讯作者：
Ruttink, Tom
影响因子：
3.6
作者：
Ferreira, Claudia de Souza;Araujo, Tomaz Henrique;Rodrigues, Maria Rita
通讯作者：
Rodrigues, Maria Rita
影响因子：
64.8
作者：
ABRAHAM, SN;SUN, DX;BEACHEY, EH
通讯作者：
BEACHEY, EH