Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses.
Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses.
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DOI：
10.1016/j.celrep.2018.03.126
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发表时间：
2018-05-01
期刊：
影响因子：
8.8
通讯作者：
Sigrist SJ
中科院分区：
文献类型：
--
作者：
Fulterer A;Andlauer TFM;Ender A;Maglione M;Eyring K;Woitkuhn J;Lehmann M;Matkovic-Rachid T;Geiger JRP;Walter AM;Nagel KI;Sigrist SJ
高通量电子显微镜已经开始揭示单个回路以及整个脑区的突触连接图谱，例如在果蝇嗅觉系统中。然而，突触小泡释放的效率、时间和频率调节在脑突触之间也高度多样化。这些特征关键取决于电压门控钙离子通道（VGCCs）和突触小泡释放机制之间的纳米级耦合距离。结合光学超分辨率显微镜和体内电生理学，我们在此表明，两种正交的支架蛋白（ELKS家族的Bruchpilot，BRP，以及Syd - 1）使特异性的（M）Unc13释放因子异构体在果蝇嗅觉系统的突触中聚集在距离VGCCs较近（BRP/Unc13A）或较远（Syd - 1/Unc13B）的位置，导致了不同的突触特征性短期可塑性形式。此外，不同突触类型之间BRP/Unc13A与Syd - 1/Unc13B的比例不同。因此，紧密耦合与松散耦合的支架蛋白/（M）Unc13模块的差异能够调节突触类型特异性的释放特征，并且“纳米分子指纹”可能识别具有特定时间特征的突触。
Fulterer等人证明，支架蛋白Bruchpilot和Syd - 1使（M）Unc13释放因子异构体在果蝇嗅觉系统中聚集在距离电压门控钙离子通道较近（BRP/Unc13A）或较远（Syd - 1/Unc13B）的位置。这些支架/释放因子“模块”在不同突触类型之间存在显著差异，从而将释放特征调节为抑制或易化。
High-throughput electron microscopy has started to reveal synaptic connectivity maps of single circuits and whole brain regions, for example, in the Drosophila olfactory system. However, efficacy, timing, and frequency tuning of synaptic vesicle release are also highly diversified across brain synapses. These features critically depend on the nano-meter-scale coupling distance between voltage-gated Ca2+ channels (VGCCs) and the synaptic vesicle release machinery. Combining light super resolution microscopy with in vivo electrophysiology, we show here that two orthogonal scaffold proteins (ELKS family Bruchpilot, BRP, and Syd-1) cluster-specific (M)Unc13 release factor isoforms either close (BRP/Unc13A) or further away (Syd-1/Unc13B) from VGCCs across synapses of the Drosophila olfactory system, resulting in different synapse-characteristic forms of short-term plasticity. Moreover, BRP/Unc13A versus Syd-1/Unc13B ratios were different between synapse types. Thus, variation in tightly versus loosely coupled scaffold protein/(M)Unc13 modules can tune synapse-type-specific release features, and “nanoscopic molecular fingerprints” might identify synapses with specific temporal features. Fulterer et al. demonstrates that the scaffold proteins Bruchpilot and Syd-1 cluster (M)Unc13 release factor isoforms either close (BRP/Unc13A) or further away (Syd-1/Unc13B) from voltage-gated Ca2+ channels in the Drosophila olfactory system. These scaffold/release factor “modules” varied significantly between different synapse types, thereby tuning release features toward depression or facilitation.
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影响因子：
64.8
作者：
Hong, Weizhe;Mosca, Timothy J.;Luo, Liqun
通讯作者：
Luo, Liqun
果蝇触角叶的突触组织及其由 Teneurins 的调节。
影响因子：
7.7
作者：
Mosca TJ;Luo L
通讯作者：
Luo L
影响因子：
25
作者：
Gruntman, Eyal;Turner, Glenn C.
通讯作者：
Turner, Glenn C.
影响因子：
7.7
作者：
Andlauer TF;Scholz-Kornehl S;Tian R;Kirchner M;Babikir HA;Depner H;Loll B;Quentin C;Gupta VK;Holt MG;Dipt S;Cressy M;Wahl MC;Fiala A;Selbach M;Schwärzel M;Sigrist SJ
通讯作者：
Sigrist SJ
影响因子：
34.7
作者：
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