Differences in gut microbiota between silkworms (Bombyx mori) reared on fresh mulberry (Morus alba var. multicaulis) leaves or an artificial diet.
Differences in gut microbiota between silkworms (Bombyx mori) reared on fresh mulberry (Morus alba var. multicaulis) leaves or an artificial diet.
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用新鲜桑叶（Morus alba var. multicaulis）或人工饲料饲养的蚕（Bombyx mori）肠道微生物群的差异
DOI：
10.1039/c8ra04627a
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发表时间：
2018-07-19
期刊：
影响因子：
3.9
通讯作者：
Xu, Shi-Qing
中科院分区：
文献类型：
--
作者：
Dong, Hui-Ling;Zhang, Sheng-Xiang;Chen, Zhuo-Hua;Tao, Hui;Li, Xue;Qiu, Jian-Feng;Cui, Wen-Zhao;Sima, Yang-Hu;Cui, Wei-Zheng;Xu, Shi-Qing
家蚕人工饲料具有广泛的应用前景，在蚕业生产中占有重要地位。然而，人工饲料饲养的蚕体虚弱和丝蛋白合成效率低的挑战尚未得到解决。在这里，我们使用高通量测序来分析桑葚饲养的5龄幼虫和人工饲料饲养的幼虫的肠道微生物群之间的差异。结果表明，在门的水平上，蓝藻门、厚壁菌门、变形菌门、拟杆菌门和放线菌门是各品系家蚕肠道的优势菌。但是，在桑葚上饲养的家蚕品系之间以及在人工饲料上饲养的家蚕品系之间，肠道微生物群中优势细菌的丰度不同，而在人工饲料上饲养的家蚕品系中肠道微生物群的多样性较低。对家蚕肠道菌群功能的预测表明，桑叶蚕品种间无显著差异，人工饲料蚕品种间差异显著。当家蚕饲料由桑葚转变为人工饲料时，家蚕肠道菌群的变化影响了宿主的营养代谢和免疫抵抗力。这些变化可能与蚕对长期食桑葚的进化历史的适应有关。
Artificial diets for silkworms have many potential applications and they are important in sericulture. However, the challenges of weak larvae and low silk protein synthesis efficiency in silkworms reared on artificial diets have not been resolved. Here, we used high-throughput sequencing to analyse the differences between the gut microbiota of 5th-instar larvae reared on mulberry leaves and larvae reared on an artificial diet. The results showed that at the phylum level, Cyanobacteria, Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria are the dominant bacteria in the intestines of silkworm larvae of all the strains. But the abundance of dominant bacteria in the gut microbiota differed between the silkworm strains that were reared on mulberry leaves, as well as between the silkworm strains that were reared on the artificial diet, while the gut microbiota diversity was lower in the silkworm strains that were reared on the artificial diet. Prediction of the functions of the gut microbiota in the hosts indicated that there was no significant difference between the silkworm strains that were reared on mulberry leaves, while there were significant differences between silkworm strains reared on the artificial diet. When the silkworm diet changed from mulberry leaves to the artificial diet, changes in gut microbiota in the silkworms affected host nutrient metabolism and immune resistance. These changes may be related to the adaptation of silkworms to their long evolutionary history of eating mulberry leaves.
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一类重要的微生物胆汁酸的生物合成途径。
影响因子：
14.8
作者：
Devlin AS;Fischbach MA
通讯作者：
Fischbach MA
DOI：
10.4269/ajtmh.2008.79.881
发表时间：
2008-12-01
影响因子：
3.3
作者：
Hillesland, Heidi;Read, Amber;Durvasula, Ravi
通讯作者：
Durvasula, Ravi
影响因子：
30.3
作者：
Backhed, Fredrik;Roswall, Josefine;Jun, Wang
通讯作者：
Jun, Wang
影响因子：
1.3
作者：
Cappellozza, L;Cappellozza, S;Sbrenna, G
通讯作者：
Sbrenna, G
影响因子：
2.2
作者：
HORIE, Y;NAKASONE, S
通讯作者：
NAKASONE, S