Proliferation and differentiation of organoid hair follicle cells co‐cultured with fat cells in collagen gel matrix culture
Proliferation and differentiation of organoid hair follicle cells co‐cultured with fat cells in collagen gel matrix culture
复制标题
胶原凝胶基质培养中与脂肪细胞共培养的类器官毛囊细胞的增殖和分化
DOI：
--
复制
发表时间：
1998
影响因子：
10.3
通讯作者：
Narisawa
中科院分区：
文献类型：
--
作者：
Misago;Toda;Sugihara;Kohda;Narisawa
利用大鼠皮肤，在三维胶原凝胶基质培养体系中，研究了脂肪细胞对类器官毛囊细胞增殖分化的影响。我们在以下三种条件下培养包埋在胶原凝胶中的类器官毛囊：对照实验（条件1）无细胞胶原凝胶；与脂肪细胞近距离共培养（条件2）；并与脂肪细胞在空间分离中共培养（条件3）。在条件1和条件3下，观察到类器官毛囊上皮细胞的生长与成纤维细胞的毛囊周围增殖有关。在条件2下，类器官毛囊细胞和成纤维细胞的增殖均受到抑制，但毛囊细胞的分化似乎有所加速。脂肪细胞被认为对毛囊周围成纤维细胞的增殖有抑制作用，这可能导致毛囊细胞增殖受到抑制，也可能更好地维持正常的毛囊结构和完整性，从而使头发类型分化得以进行。脂肪细胞对毛囊分化的直接加速作用可能也是原因之一。在生理状态下（与角质形成细胞在胶原凝胶上共培养），在条件1和条件2下观察到类似的结果。2和3条件下不同的结果可能是由于两种可能:要么可溶性因子的浓度梯度从脂肪细胞释放,作用于毛囊细胞或perifollicular成纤维细胞增殖抑制剂,造成结果的差异,或直接接触瀑样毛囊细胞和脂肪细胞可能影响脂肪细胞的加速作用于毛囊细胞的分化。
Using rat skin, we studied the influence of fat cells on the proliferation and differentiation of organoid hair follicle cells in a three‐dimensional collagen gel matrix culture system. We cultured organoid hair follicles embedded in collagen gel under each of the following three conditions: cell‐free collagen gel for control experiments (condition 1); co‐culture with fat cells in close apposition (condition 2); and co‐culture with fat cells in spatial separation (condition 3). Outgrowths of epithelial cells from the organoid hair follicles associated with perifollicular proliferation of fibroblasts were observed under conditions 1 and 3. Under condition 2, proliferation of both organoid hair follicle cells and fibroblasts was inhibited, but differentiation of the hair follicle cells appeared to be accelerated. Fat cells are considered to have an inhibitory effect on the proliferation of perifollicular fibroblasts, which might have resulted in the inhibition of hair follicle cell proliferation and also in the better maintenance of normal follicular structure and integrity, allowing for hair‐type differentiation to proceed. A direct accelerating effect of fat cells on hair follicle differentiation may also have been responsible. In a physiological state (co‐culture with keratinocytes on the collagen gel), similar results were observed under conditions 1 and 2. The different findings under conditions 2 and 3 may be due to either of two possibilities: either the concentration gradient of the soluble factors released from fat cells, acting on either the hair follicle cells or the perifollicular fibroblasts as an inhibitor of proliferation, caused the difference in the results, or direct contact between the organoid hair follicle cells and fat cells may have influenced the accelerating effect of fat cells on the differentiation of hair follicle cells.
DOI：
10.1016/s0021-9258(19)50409-4
发表时间：
1992-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. T. White;D. Damm;N. Hancock;B. Rosen;B. Lowell;P. Usher;J. Flier;B. Spiegelman
通讯作者：
R. T. White;D. Damm;N. Hancock;B. Rosen;B. Lowell;P. Usher;J. Flier;B. Spiegelman
肿瘤坏死因子-α的脂肪表达--在与肥胖相关的胰岛素抵抗中的直接作用
影响因子：
56.9
作者：
HOTAMISLIGIL, GS;SHARGILL, NS;SPIEGELMAN, BM
通讯作者：
SPIEGELMAN, BM