Revealing the transfer pathways of cyanobacterial-fixed N into the boreal forest through the feather-moss microbiome.
Revealing the transfer pathways of cyanobacterial-fixed N into the boreal forest through the feather-moss microbiome.
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DOI：
10.3389/fpls.2022.1036258
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发表时间：
2022
影响因子：
5.6
通讯作者：
DeLuca, Thomas H.
中科院分区：
文献类型：
--
作者：
Arroniz-Crespo, Maria;Bougoure, Jeremy;Murphy, Daniel, V;Cutler, Nick A.;Souza-Egipsy, Virginia;Chaput, Dominique L.;Jones, Davey L.;Ostle, Nicholas;Wade, Stephen C.;Clode, Peta L.;DeLuca, Thomas H.
关键词：
苔藓生物固氮是北方森林生态系统新氮(N)的最大输入之一；然而，揭示新固定N在苔藓圈(即苔藓植物及其相关生物)中的命运仍不确定。在这里，我们结合15N示踪剂、高分辨率二次离子质谱仪(NanoSIMS)和细菌、真菌和重氮营养群落的分子调查，确定了羽状苔藓(Pleurozium Schreberi)及其相关微生物群内新固定的氮的来源和转移途径。NanoSIMS图像显示，新固定的来自蓝藻的氮气被整合到苔藓组织以及相关的细菌、真菌和微藻中。这些图像表明，先前关于新固定的氮气被隔离在苔藓组织中并仅通过分解释放的假设是不正确的。我们首次提供了固定在羽藓中的氮气进入北方森林生态系统(即通过其微生物群)的新途径的经验证据，并讨论了其对更广泛的生态系统功能的影响。
Biological N2 fixation in feather-mosses is one of the largest inputs of new nitrogen (N) to boreal forest ecosystems; however, revealing the fate of newly fixed N within the bryosphere (i.e. bryophytes and their associated organisms) remains uncertain. Herein, we combined 15N tracers, high resolution secondary ion mass-spectrometry (NanoSIMS) and a molecular survey of bacterial, fungal and diazotrophic communities, to determine the origin and transfer pathways of newly fixed N2 within feather-moss (Pleurozium schreberi) and its associated microbiome. NanoSIMS images reveal that newly fixed N2, derived from cyanobacteria, is incorporated into moss tissues and associated bacteria, fungi and micro-algae. These images demonstrate that previous assumptions that newly fixed N2 is sequestered into moss tissue and only released by decomposition are not correct. We provide the first empirical evidence of new pathways for N2 fixed in feather-mosses to enter the boreal forest ecosystem (i.e. through its microbiome) and discuss the implications for wider ecosystem function.
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影响因子：
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Bragina A;Maier S;Berg C;Müller H;Chobot V;Hadacek F;Berg G
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