The genome of the brown alga Ectocarpus siliculosus contains a series of viral DNA pieces, suggesting an ancient association with large dsDNA viruses.
The genome of the brown alga Ectocarpus siliculosus contains a series of viral DNA pieces, suggesting an ancient association with large dsDNA viruses.
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棕色藻类硅藻的基因组包含一系列病毒DNA碎片，表明与大型DSDNA病毒的古老关联。
DOI：
10.1186/1471-2148-8-110
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发表时间：
2008-04-12
影响因子：
3.4
通讯作者：
Boland, Wilhelm
中科院分区：
文献类型：
--
作者：
Delaroque, Nicolas;Boland, Wilhelm
硅壳香树病毒1（Ectocarpus siliculosus virus-1，EsV-1）是一种溶源性双链DNA病毒，属于核质大DNA病毒（Nucleocytoplasmic Large DNA Virus，NCLDV）超家族，主要感染硅壳香树（Ectocarpus siliculosus）。以前的研究表明，病毒基因组整合到宿主DNA中。为了寻找病毒基因组的整合位点，使用标记的EsV-1 DNA筛选来自EsV-1感染的藻类的基因组文库。对其中的一些片段进行了测序和分析。分析显示，藻类基因组被一个与EsV-1 DNA序列具有高度同一性的病毒序列拷贝分裂。这些片段散布有DNA重复序列、假基因和编码参与DNA复制、整合和转座的产物的基因。这些基因产物中的一些不由EsV-1编码，但存在于NCLDV家族的其他成员的基因组中。进一步的分析表明，Ectocarpus藻类基因组含有大dsDNA病毒基因组整合的痕迹;该基因组可能是现存NCLDV基因组的祖先。此外，一些证据表明EsV-1基因组可能起源于这些病毒DNA片段，这意味着存在复杂的整合和重组系统。一种类似于新的酪氨酸重组酶的蛋白质可能是该系统的关键酶。我们的研究结果支持这样的假设，即一些dsDNA病毒是单系的，主要通过基因组减少进化。此外，我们假设，可能已经开发了一个原始的复制系统。
Ectocarpus siliculosus virus-1 (EsV-1) is a lysogenic dsDNA virus belonging to the super family of nucleocytoplasmic large DNA viruses (NCLDV) that infect Ectocarpus siliculosus, a marine filamentous brown alga. Previous studies indicated that the viral genome is integrated into the host DNA. In order to find the integration sites of the viral genome, a genomic library from EsV-1-infected algae was screened using labelled EsV-1 DNA. Several fragments were isolated and some of them were sequenced and analyzed in detail. Analysis revealed that the algal genome is split by a copy of viral sequences that have a high identity to EsV-1 DNA sequences. These fragments are interspersed with DNA repeats, pseudogenes and genes coding for products involved in DNA replication, integration and transposition. Some of these gene products are not encoded by EsV-1 but are present in the genome of other members of the NCLDV family. Further analysis suggests that the Ectocarpus algal genome contains traces of the integration of a large dsDNA viral genome; this genome could be the ancestor of the extant NCLDV genomes. Furthermore, several lines of evidence indicate that the EsV-1 genome might have originated in these viral DNA pieces, implying the existence of a complex integration and recombination system. A protein similar to a new class of tyrosine recombinases might be a key enzyme of this system. Our results support the hypothesis that some dsDNA viruses are monophyletic and evolved principally through genome reduction. Moreover, we hypothesize that phaeoviruses have probably developed an original replication system.
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