Hsf and Hsp gene families in Populus: genome-wide identification, organization and correlated expression during development and in stress responses.
Hsf and Hsp gene families in Populus: genome-wide identification, organization and correlated expression during development and in stress responses.
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DOI：
10.1186/s12864-015-1398-3
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发表时间：
2015-03-14
期刊：
影响因子：
4.4
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Zhang J;Liu B;Li J;Zhang L;Wang Y;Zheng H;Lu M;Chen J
热休克蛋白（Heat shock proteins，Hsps）是参与许多正常细胞过程和应激反应的分子伴侣，热休克因子（Heat shock factors，Hsfs）是Hsps的转录激活因子。Hsfs和Hsps在各种生物过程中广泛协调。虽然Hsfs和Hsps在拟南芥中的胁迫响应中的作用已经得到很好的表征，但它们在经历各种环境胁迫的多年生木本植物中的作用仍然不清楚。本文对杨树Hsf和Hsp家族进行了全面的鉴定和分析。在胡杨中，我们确定了42个旁系同源对，66.7%来自全基因组重复。各亚家族的基因结构和基序组成相对保守。基因芯片和实时荧光定量RT-PCR分析表明，大多数杨树Hsf和Hsp基因的差异表达后，暴露于各种压力。根据Hsf和Hsp基因的表达情况构建了一个共表达网络。通过瞬时过表达和随后的qPCR分析验证协调关系。综合分析表明，不同套PtHsps下游的特定PtHsfs和功能研究，旨在揭示这些家庭在白杨发展和胁迫反应的作用提供了基础。本文的在线版本（doi：10.1186/s12864-015-1398-3）包含补充材料，可供授权用户使用。
Heat shock proteins (Hsps) are molecular chaperones that are involved in many normal cellular processes and stress responses, and heat shock factors (Hsfs) are the transcriptional activators of Hsps. Hsfs and Hsps are widely coordinated in various biological processes. Although the roles of Hsfs and Hsps in stress responses have been well characterized in Arabidopsis, their roles in perennial woody species undergoing various environmental stresses remain unclear. Here, a comprehensive identification and analysis of Hsf and Hsp families in poplars is presented. In Populus trichocarpa, we identified 42 paralogous pairs, 66.7% resulting from a whole genome duplication. The gene structure and motif composition are relatively conserved in each subfamily. Microarray and quantitative real-time RT-PCR analyses showed that most of the Populus Hsf and Hsp genes are differentially expressed upon exposure to various stresses. A coexpression network between Populus Hsf and Hsp genes was generated based on their expression. Coordinated relationships were validated by transient overexpression and subsequent qPCR analyses. The comprehensive analysis indicates that different sets of PtHsps are downstream of particular PtHsfs and provides a basis for functional studies aimed at revealing the roles of these families in poplar development and stress responses. The online version of this article (doi:10.1186/s12864-015-1398-3) contains supplementary material, which is available to authorized users.
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