Variation in MicroRNA Expression Profile of Uterine Leiomyoma with Endometrial Cavity Distortion and Endometrial Cavity Non-Distortion.
Variation in MicroRNA Expression Profile of Uterine Leiomyoma with Endometrial Cavity Distortion and Endometrial Cavity Non-Distortion.
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DOI：
10.3390/ijms19092524
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发表时间：
2018-08-25
影响因子：
5.6
通讯作者：
Suh CS
中科院分区：
文献类型：
--
作者：
Kim YJ;Kim YY;Shin JH;Kim H;Ku SY;Suh CS
microRNA（miRNA）在子宫平滑肌瘤（UL）细胞中的表达谱不同于正常子宫肌层（UM）细胞。UL细胞对子宫容受性的影响可能因其扭曲子宫内膜腔的能力而异。然而，子宫内膜腔扭曲平滑肌瘤（ECDL）和子宫内膜腔非扭曲平滑肌瘤（ECNDL）细胞之间的miRNA表达谱的变化仍然未知。本研究旨在阐明子宫内膜异位症患者子宫从UL患者（n = 26）中进行盆腔镜子宫肌瘤切除术，以获得UL组织样本及其相应的正常UM组织（匹配），其中ECNDL和ECDL女性分别为15和11。使用qRT-PCR将UL细胞中hsa-miR-15 b、-29 a、-29 b、-29 c、-197和-200c以及候选靶基因的相对表达与匹配的UM细胞中的相对表达进行比较，以评估它们引起ECD的能力。采用原位杂交技术分析UL组织中miRNAs和靶基因的空间表达。在用miRNA的模拟物和抑制剂转染UL细胞后，使用qPCR分析靶基因表达。与UM细胞相比，UL细胞中miR-15 b的相对表达水平上调，miR-29 a、-29 b、-29 c、-197和-200 c的相对表达水平下调。与UM细胞相比，UL细胞中孕激素受体、雌激素受体和基质金属蛋白酶（MMPs）的相对表达水平上调。与ECNDL细胞相比，ECDL细胞中miR-29 c和-200c的相对表达水平下调，雌激素受体、MMPs和组织金属蛋白酶抑制剂（TIMPs）的相对表达水平上调。UL细胞中miRNAs的表达谱随着子宫内膜腔变形的发生或不发生而变化。UL的生化特性可能是由miRNA调节，以改变其对子宫结构稳态的影响。
The expression profile of microRNA (miRNA) in uterine leiomyoma (UL) cells is different from that in normal uterine myometrial (UM) cells. The effect of UL cells on uterine receptivity might vary according to their ability to distort the uterine endometrial cavity. However, the variation in miRNA expression profiles between endometrial cavity-distorting leiomyoma (ECDL) and endometrial cavity non-distorting leiomyoma (ECNDL) cells remains unknown. This study aimed to elucidate whether the expression profile of miRNAs in ECDL cells is dissimilar to that of ECNDL cells in uterus. Pelviscopic myomectomy was performed to obtain tissue samples of UL and their corresponding normal UM tissues (matched) from patients with UL (n = 26), among whom women with ECNDL and ECDL numbered 15 and 11, respectively. The relative expression of hsa-miR-15b, -29a, -29b, -29c, -197, and -200c as well as the candidate target genes in UL cells was compared to those in the matched UM cells using qRT-PCR to assess their ability to cause ECD. The spatial expression of miRNAs and target genes in the UL tissues was analyzed using in situ hybridization. Target gene expression was analyzed using qPCR after transfection with the mimics and inhibitors of miRNAs in UL cells. The relative expression level of miR-15b was upregulated, and the relative expression levels of miR-29a, -29b, -29c, -197, and -200c were downregulated in UL cells compared to those in UM cells. The relative expression levels of progesterone receptor, estrogen receptor, and matrix metalloproteinases (MMPs) were upregulated in UL cells compared to those in UM cells. The relative expression levels of miR-29c and -200c were downregulated, and the relative expression levels of estrogen receptor, MMPs and tissue inhibitors of metalloproteinases (TIMPs) were upregulated in ECDL cells compared to those in ECNDL cells. The expression profile of miRNAs in UL cells varied with respect to the occurrence or absence of endometrial cavity distortion. The biochemical properties of UL might be regulated by miRNAs in order to alter their effect on structural homeostasis of the uterus.
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