Fecal microbiota and metabolome of children with autism and pervasive developmental disorder not otherwise specified.
Fecal microbiota and metabolome of children with autism and pervasive developmental disorder not otherwise specified.
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DOI：
10.1371/journal.pone.0076993
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Francavilla R
中科院分区：
文献类型：
--
作者：
De Angelis M;Piccolo M;Vannini L;Siragusa S;De Giacomo A;Serrazzanetti DI;Cristofori F;Guerzoni ME;Gobbetti M;Francavilla R
本研究旨在调查患有未明确的广泛性发育障碍 (PDD-NOS) 和自闭症 (AD) 儿童与健康儿童 (HC) 的粪便微生物群和代谢组。对 16S rDNA 和 16S rRNA 进行细菌标签编码的 FLX-钛扩增子焦磷酸测序 (bTEFAP) 分析，分别确定细菌总数 (16S rDNA) 和代谢活跃细菌 (16S rRNA)。三组儿童主要细菌门（厚壁菌门、拟杆菌门、梭杆菌门和疣微菌门）发生显着变化（P<0.05）。根据稀疏度、Chao 和 Shannon 多样性指数估计，AD 儿童的微生物多样性最高。根据 16S-rRNA 和培养依赖性数据，PDD-NOS 和 HC 儿童的粪便样本中粪杆菌和瘤胃球菌的含量最高。 Caloramator、Sarcina 和 Clostridium 属在 AD 儿童中含量最高。与 HC 相比，PDD-NOS 中毛螺菌科的组成也有所不同，尤其是 AD 儿童。除西雷真杆菌外，AD儿童粪便样本中的真杆菌科细菌含量最低。 PDD-NOS或AD儿童中Bacteroidetes属以及部分Alistipes和Akkermansia的水平几乎最高，几乎所有已鉴定的Sutterellaceae和Enterobacteriaceae在AD儿童中水平最高。与正常儿童相比，AD 患者的双歧杆菌种类减少。主坐标规范判别分析显示，PDD-NOS，尤其是 AD 儿童粪便样本中游离氨基酸和挥发性有机化合物的水平受到显着影响。如果 AD 和 PDD-NOS 以及 HC 儿童之间的肠道微生物群差异是自闭症的伴随原因之一或后果之一，那么它们可能对特定的诊断测试和/或治疗和预防产生影响。
This study aimed at investigating the fecal microbiota and metabolome of children with Pervasive Developmental Disorder Not Otherwise Specified (PDD-NOS) and autism (AD) in comparison to healthy children (HC). Bacterial tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) of the 16S rDNA and 16S rRNA analyses were carried out to determine total bacteria (16S rDNA) and metabolically active bacteria (16S rRNA), respectively. The main bacterial phyla (Firmicutes, Bacteroidetes, Fusobacteria and Verrucomicrobia) significantly (P<0.05) changed among the three groups of children. As estimated by rarefaction, Chao and Shannon diversity index, the highest microbial diversity was found in AD children. Based on 16S-rRNA and culture-dependent data, Faecalibacterium and Ruminococcus were present at the highest level in fecal samples of PDD-NOS and HC children. Caloramator, Sarcina and Clostridium genera were the highest in AD children. Compared to HC, the composition of Lachnospiraceae family also differed in PDD-NOS and, especially, AD children. Except for Eubacterium siraeum, the lowest level of Eubacteriaceae was found on fecal samples of AD children. The level of Bacteroidetes genera and some Alistipes and Akkermansia species were almost the highest in PDD-NOS or AD children as well as almost all the identified Sutterellaceae and Enterobacteriaceae were the highest in AD. Compared to HC children, Bifidobacterium species decreased in AD. As shown by Canonical Discriminant Analysis of Principal Coordinates, the levels of free amino acids and volatile organic compounds of fecal samples were markedly affected in PDD-NOS and, especially, AD children. If the gut microbiota differences among AD and PDD-NOS and HC children are one of the concomitant causes or the consequence of autism, they may have implications regarding specific diagnostic test, and/or for treatment and prevention.
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