207-nm UV light - a promising tool for safe low-cost reduction of surgical site infections. I: in vitro studies.
207-nm UV light - a promising tool for safe low-cost reduction of surgical site infections. I: in vitro studies.
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DOI：
10.1371/journal.pone.0076968
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Brenner DJ
中科院分区：
文献类型：
--
作者：
Buonanno M;Randers-Pehrson G;Bigelow AW;Trivedi S;Lowy FD;Spotnitz HM;Hammer SM;Brenner DJ
0.5% 到 10% 的清洁手术会导致手术部位感染，而降低这一比率的尝试收效甚微。杀菌紫外线灯具有 200 至 400 nm 的宽波长光谱，是针对耐药和药物敏感细菌的有效杀菌选择，但对患者和工作人员构成健康危害。相比之下，由于其穿透力有限，~200 nm 远紫外光预计可有效杀死细菌，但不会像传统杀菌紫外线照射那样对皮肤和眼睛造成人体健康危害。这项工作的目的是测试基于生物物理学的假设，即~200 nm 紫外线对细菌具有显着的细胞毒性，但对分离的或组织内的人类细胞具有最小的细胞毒性或致突变性。使用了 Kr-Br 准分子灯，可产生 207 nm 的紫外线，并配有滤光器以去除较高波长的成分。与传统广谱 254 nm 紫外线杀菌灯的结果进行了比较。首先，生成了耐甲氧西林金黄色葡萄球菌 (MRSA) 细菌以及正常人成纤维细胞的细胞失活与紫外线注量数据。其次，对于入射到 3D 人体皮肤组织上的两种紫外线辐射，测量了主要与紫外线相关的诱变前 DNA 损伤（环丁烷嘧啶二聚体和 6-4 光产物）的产量。我们发现 207 nm 紫外线可以有效杀死 MRSA，但与传统的杀菌紫外线灯不同，它对人体细胞的细胞杀伤作用很小。在 3D 人体皮肤模型中，207 nm 紫外线几乎不会产生与突变前紫外线相关的 DNA 损伤，这与传统杀菌紫外线灯产生的显着产量形成鲜明对比。正如基于生物物理学考虑的预测，207 nm 光可以有效杀死细菌，但似乎对人体细胞没有显着的细胞毒性或诱变性。如果使用得当，207 nm 光可能具有安全且廉价地降低手术部位感染率的潜力，包括耐药性来源的感染率。
0.5% to 10% of clean surgeries result in surgical-site infections, and attempts to reduce this rate have had limited success. Germicidal UV lamps, with a broad wavelength spectrum from 200 to 400 nm are an effective bactericidal option against drug-resistant and drug-sensitive bacteria, but represent a health hazard to patient and staff. By contrast, because of its limited penetration, ∼200 nm far-UVC light is predicted to be effective in killing bacteria, but without the human health hazards to skin and eyes associated with conventional germicidal UV exposure. The aim of this work was to test the biophysically-based hypothesis that ∼200 nm UV light is significantly cytotoxic to bacteria, but minimally cytotoxic or mutagenic to human cells either isolated or within tissues. A Kr-Br excimer lamp was used, which produces 207-nm UV light, with a filter to remove higher-wavelength components. Comparisons were made with results from a conventional broad spectrum 254-nm UV germicidal lamp. First, cell inactivation vs. UV fluence data were generated for methicillin-resistant S. aureus (MRSA) bacteria and also for normal human fibroblasts. Second, yields of the main UV-associated pre-mutagenic DNA lesions (cyclobutane pyrimidine dimers and 6-4 photoproducts) were measured, for both UV radiations incident on 3-D human skin tissue. We found that 207-nm UV light kills MRSA efficiently but, unlike conventional germicidal UV lamps, produces little cell killing in human cells. In a 3-D human skin model, 207-nm UV light produced almost no pre-mutagenic UV-associated DNA lesions, in contrast to significant yields induced by a conventional germicidal UV lamp. As predicted based on biophysical considerations, 207-nm light kills bacteria efficiently but does not appear to be significantly cytotoxic or mutagenic to human cells. Used appropriately, 207-nm light may have the potential for safely and inexpensively reducing surgical-site infection rates, including those of drug-resistant origin.
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影响因子：
2.9
作者：
Kreusch, S;Schwedler, S;Horn, A
通讯作者：
Horn, A
影响因子：
3.4
作者：
KOCHEVAR, IE;WALSH, AA;MIRRO, J
通讯作者：
MIRRO, J
DOI：
10.1302/0301-620x.73b5.1894672
发表时间：
1991-09-01
影响因子：
--
作者：
BERG, M;BERGMAN, BR;HOBORN, J
通讯作者：
HOBORN, J
影响因子：
3.4
作者：
JOSE, JG;PITTS, DG
通讯作者：
PITTS, DG
影响因子：
11.8
作者：
Lessa, Fernanda C.;Mu, Yi;Fridkin, Scott K.
通讯作者：
Fridkin, Scott K.