Does unrestrained single-chamber plethysmography provide a valid assessment of airway responsiveness in allergic BALB/c mice?
Does unrestrained single-chamber plethysmography provide a valid assessment of airway responsiveness in allergic BALB/c mice?
复制标题
无限制单腔体积描记法是否可以有效评估过敏性 BALB/c 小鼠的气道反应性？
DOI：
10.1186/1465-9921-10-61
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发表时间：
2009-07-03
影响因子：
5.8
通讯作者：
Zhong N
中科院分区：
文献类型：
--
作者：
Zhang Q;Lai K;Xie J;Chen G;Zhong N
背景无约束体积描记法由于其易于使用和能够测量清醒动物的气道反应性而被用于监测支气管收缩。然而，它的可靠性仍然存在争议。Objectiveinvestigate如果不受限制的体积描记法可以提供一个有效的解释过敏BALB/c mice.MethodsOvalbumin致敏BALB/c小鼠的气道反应性随机接受单剂量卵清蛋白的挑战（OVA-1D组）或三剂量卵清蛋白的挑战（OVA-3D组）。将OVA-1D组进一步分为OVA-1D-I（以肺阻力为反应性指标，有创性测量）和OVA-1D-N组（以Penh为反应性指标，无创性测量）。同样，根据上述方法将OVA-3D组分为OVA-3D-I组和OVA-3D-N组。对照组用生理盐水致敏和激发。取支气管肺泡灌洗液，进行气道炎症的气道组织病理学评价。结果与对照组相比，OVA-1D-N组气道反应性显著增加（P < 0.05），而OVA-1D-I组气道反应性无显著差异（P> 0.05）。OVA-3D-I组和OVA-3D-N组的反应性均高于对照组（P < 0.05）。所有卵清蛋白免疫组的鼻粘膜均被嗜酸性细胞浸润。过敏组的喷嚏或揉鼻现象较对照组更为频繁。结论Penh不能作为气道阻力的替代指标。侵入性测量是特定于下气道的。Penh测量（作为筛查程序进行）必须通过下气道特异性直接侵入性测量来确认，以评估下气道反应性。
BackgroundUnrestrained plethysmography has been used to monitor bronchoconstriction because of its ease of use and ability to measure airway responsiveness in conscious animals. However, its reliability remains controversial.ObjectiveTo investigate if unrestrained plethysmography could provide a valid interpretation of airway responsiveness in allergic BALB/c mice.MethodsOvalbumin sensitized BALB/c mice were randomized to receive either a single-dose Ovalbumin challenge (OVA-1D group) or a three-dose Ovalbumin challenge (OVA-3D group). The OVA-1D group was further divided into OVA-1D-I (measured invasively, using lung resistance as the index of responsiveness) and OVA-1D-N group (measured non-invasively, using Penh as the index of responsiveness). Similarly the OVA-3D group was divided into OVA-3D-I and OVA-3D-N groups based on the above methods. The control groups were sensitized and challenged with normal saline. Bronchial alveolar lavage fluid was taken and airway histopathology was evaluated for airway inflammation. Nasal responsiveness was tested with histamine challenge.ResultsCompared with controls, a significant increase in airway responsiveness was shown in the OVA-1D-N group (P < 0.05) but not in the OVA-1D-I group. Both OVA-3D-I and OVA-3D-N groups showed higher responsiveness than their controls (P < 0.05). The nasal mucosa was infiltrated by eosinophic cells in all Ovalbumin immunized groups. Sneezing or nasal rubbing in allergic groups appeared more frequent than that in the control groups.ConclusionPenh can not be used as a surrogate for airway resistance. The invasive measurement is specific to lower airway. Penh measurement (done as a screening procedure), must be confirmed by a direct invasive measurement specific to lower airway in evaluating lower airway responsiveness.
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影响因子：
3.3
作者：
Lundblad, LKA;Irvin, CG;Bates, JHT
通讯作者：
Bates, JHT
影响因子：
5
作者：
Nakaya, Muneo;Dohi, Makoto;Kaga, Kimitaka
通讯作者：
Kaga, Kimitaka
影响因子：
15.9
作者：
Schwarze, J;Hamelmann, E;Gelfand, EW
通讯作者：
Gelfand, EW
DOI：
10.1164/ajrccm.156.3.9606031
发表时间：
1997-09-01
影响因子：
24.7
作者：
Hamelmann, E;Schwarze, J;Gelfand, EW
通讯作者：
Gelfand, EW
影响因子：
9.6
作者：
Brightling, CE
通讯作者：
Brightling, CE