Factors influencing tumor response to photodynamic therapy sensitized by intratumor administration of methylene blue.
Factors influencing tumor response to photodynamic therapy sensitized by intratumor administration of methylene blue.
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DOI：
10.1002/lsm.20962
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发表时间：
2010-10
影响因子：
2.4
通讯作者：
Foster, Thomas H.
中科院分区：
文献类型：
--
作者：
Baran, Timothy M.;Giesselman, Benjamin R.;Hu, Rui;Biel, Merrill A.;Foster, Thomas H.
我们在小鼠肿瘤模型中检测了亚甲蓝(MB)介导光动力疗法(PDT)对肿瘤的反应。目的是研究药物-光间隔(DLI)、注射载体和注量对肿瘤破坏的影响。荧光光谱和反射光谱加深了我们的理解。将EMT6肿瘤细胞接种于雌性BALB/c小鼠背部皮下，生长至直径约4 mm。小鼠单点注射35μL，瘤内注射亚甲基蓝500μg/m L，分别用水或5%乙醇-5%乳油-90%生理盐水给药。以60 mW/cm2的注量率，立即或在DLI后1小时开始PDT。每只动物接受240或480J/cm2的剂量。在辐照前和辐照过程中捕获荧光光谱和反射光谱。由以乳油为基础的载体、0 DLI和480J/cm2的剂量组成的方案是最有效的，根据PDT后90天没有肿瘤证据来衡量，治愈率为55%。使用带有这种通量的水载体和DLI将治愈率降低到20%。将剂量降低到240J/cm2同样会降低0和1h DLIS的治疗效果。单变量COX比例风险分析确定，剂量增加、0与1小时DLI、乳油与水载体是肿瘤长期控制的高度显著的独立预测因素(P<；0.01)。模型选择的多变量分析显示，注量和注射工具是生存风险的最好预测因素。体内荧光光谱显示，MB荧光在2-h暗时间间隔内单调减弱，但经照射后恢复。反射光谱显示，在该注入浓度的亚甲基蓝显着衰减处理光束。敏化剂传递载体、药物-光间隔和光通量对MB介导的光动力疗法的肿瘤反应有显著影响。
We examined tumor response to methylene blue (MB)-mediated photodynamic therapy (PDT) in a murine tumor model. The goal was to investigate the effects of drug-light interval (DLI), injection vehicle, and fluence on tumor destruction. Fluorescence and reflectance spectroscopy informed our understanding. EMT6 tumor cells were implanted intradermally on the backs of female BALB/c mice and grown to ~ 4-mm diameter. Mice were given a 35 μL, single site, intratumor injection of 500 μg/mL MB administered in either a water or a 5% ethanol-5% Cremophor-90% saline vehicle. PDT was begun either immediately or after a 1-hour DLI with a fluence rate of 60 mW/cm2. Each animal received a fluence of 240 or 480 J/cm2. Fluorescence and reflectance spectra were captured before and during irradiation. A protocol consisting of the Cremophor-based vehicle, 0 DLI, and a fluence of 480 J/cm2 was the most effective, with a 55% cure rate as measured by no evidence of tumor 90 days after PDT. Use of the water vehicle with this fluence and DLI reduced the cure rate to 20%. Reducing the fluence to 240 J/cm2 similarly reduced treatment efficacy with 0 and 1-h DLIs. Univariate Cox proportional hazards analysis identified increased fluence, 0 vs. 1-h DLI, and the Cremophor vs. water vehicle as highly significant independent predictors of long term tumor control (p < 0.01 in each case). Multivariate analysis with model selection revealed fluence and injection vehicle as the best predictors of survival hazards. Fluorescence spectroscopy in vivo showed that MB fluorescence decreased monotonically during a 2-h dark interval but was restored by irradiation. Reflectance spectroscopy revealed that MB at this injected concentration attenuates the treatment beam significantly. Sensitizer delivery vehicle, drug-light interval, and fluence contribute significantly to the tumor response to MB-mediated PDT.
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DOI：
10.1073/pnas.93.15.7446
发表时间：
1996-07-23
影响因子：
11.1
作者：
Jockusch, S;Lee, D;Leonard, EF
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影响因子：
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作者：
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DOI：
10.1016/j.jphotobiol.2003.06.002
发表时间：
2003-10-15
影响因子：
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作者：
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作者：
Tardivo, Joao P.;Del Giglio, Auro;Baptista, Mauricio S.
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DOI：
10.1002/hed.10372
发表时间：
2004-03-01
影响因子：
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