SERS-based plasmonic nanobiosensing in single living cells.

SERS-based plasmonic nanobiosensing in single living cells.
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DOI:
10.1007/s00216-008-2521-y
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发表时间:
2009-02
影响因子:
4.3
通讯作者:
Vo-Dinh, Tuan
Vo-Dinh, Tuan
中科院分区:
化学2区
文献类型:
--
作者:
Scaffidi, Jonathan P.;Gregas, Molly K.;Seewaldt, Victoria;Vo-Dinh, Tuan

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在本文中,我们描述了一种ph敏感等离子体活性光纤纳米探针的开发和应用,适用于使用表面增强拉曼散射(SERS)检测单个活细胞的细胞内生物分析。通过测量HMEC-15/hTERT永生化的“正常”人乳腺上皮细胞和PC-3人前列腺癌细胞的细胞内pH值,证明了基于sers的光纤纳米探针的有效性和实用性。结果表明,光纤纳米探针的插入和询问为单细胞水平的细胞微环境监测提供了一种敏感和选择性的手段。
In this paper, we describe the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The effectiveness and usefulness of SERS-based fiber-optic nanoprobes are illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized “normal” human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor cellular microenvironments at the single cell level.
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