Topically applied ZnO nanoparticles suppress allergen induced skin inflammation but induce vigorous IgE production in the atopic dermatitis mouse model.
Topically applied ZnO nanoparticles suppress allergen induced skin inflammation but induce vigorous IgE production in the atopic dermatitis mouse model.
复制标题
DOI：
10.1186/s12989-014-0038-4
复制
发表时间：
2014-08-14
影响因子：
10
通讯作者：
Alenius H
中科院分区：
文献类型：
--
作者：
Ilves M;Palomäki J;Vippola M;Lehto M;Savolainen K;Savinko T;Alenius H
ZnO等金属氧化物纳米颗粒用于防晒霜，因为它们改善了它们对导致皮肤损伤和皮肤癌的紫外线的光学特性。然而，在防晒霜中用作紫外线过滤剂并应用于皮肤的颗粒的危险特性仍然没有被表征。在这里，我们研究了不同尺寸的ZnO颗粒是否能够穿透损伤的皮肤和损伤的过敏性皮肤在小鼠特应性皮炎模型后，反复局部应用ZnO颗粒。将纳米尺寸的ZnO（nZnO）和大尺寸的ZnO（bZnO）应用于机械损伤的小鼠皮肤，有或没有过敏原/超抗原致敏。过敏原/超抗原致敏引起皮肤局部炎症和过敏，并用作特应性皮炎（AD）的疾病模型。我们的研究结果表明，只有nZnO能够到达过敏性皮肤的深层，而bZnO停留在受损和过敏性皮肤的上层。此外，这两种类型的颗粒都可以减轻AD小鼠模型中诱导的局部皮肤炎症;然而，nZnO具有更高的抑制局部效应的潜力。此外，特别是nZnO诱导IgE抗体的全身产生，这是局部应用的nZnO的过敏促进佐剂特性的证据。这些结果提供了关于化妆品中常用的金属氧化物纳米颗粒的新的危害表征数据，并为这些材料在受伤皮肤中的皮肤暴露和危害评估提供了新的见解。
Metal oxide nanoparticles such as ZnO are used in sunscreens as they improve their optical properties against the UV-light that causes dermal damage and skin cancer. However, the hazardous properties of the particles used as UV-filters in the sunscreens and applied to the skin have remained uncharacterized. Here we investigated whether different sized ZnO particles would be able to penetrate injured skin and injured allergic skin in the mouse atopic dermatitis model after repeated topical application of ZnO particles. Nano-sized ZnO (nZnO) and bulk-sized ZnO (bZnO) were applied to mechanically damaged mouse skin with or without allergen/superantigen sensitization. Allergen/superantigen sensitization evokes local inflammation and allergy in the skin and is used as a disease model of atopic dermatitis (AD). Our results demonstrate that only nZnO is able to reach into the deep layers of the allergic skin whereas bZnO stays in the upper layers of both damaged and allergic skin. In addition, both types of particles diminish the local skin inflammation induced in the mouse model of AD; however, nZnO has a higher potential to suppress the local effects. In addition, especially nZnO induces systemic production of IgE antibodies, evidence of allergy promoting adjuvant properties for topically applied nZnO. These results provide new hazard characterization data about the metal oxide nanoparticles commonly used in cosmetic products and provide new insights into the dermal exposure and hazard assessment of these materials in injured skin.
登录
查看更多内容
影响因子：
6.5
作者：
Savinko, Terhi;Matikainen, Sampsa;Alenius, Harri
通讯作者：
Alenius, Harri
影响因子：
14.2
作者：
Simon, D;Vassina, E;Simon, HU
通讯作者：
Simon, HU
影响因子：
--
作者：
Wang, Steven Q.;Tooley, Ian R.
通讯作者：
Tooley, Ian R.
影响因子：
2.7
作者：
Picardi, Angelo;Lega, Ilaria;Tarolla, Emanuele
通讯作者：
Tarolla, Emanuele
影响因子：
3.7
作者：
Lin, Lynlee L.;Grice, Jeffrey E.;Prow, Tarl W.
通讯作者：
Prow, Tarl W.