The Mildew Resistance Locus O 4 Interacts with CaM/CML and Is Involved in Root Gravity Response.
The Mildew Resistance Locus O 4 Interacts with CaM/CML and Is Involved in Root Gravity Response.
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防霉基因座 O 4 与 CaM/CML 相互作用并参与根重力响应
DOI：
10.3390/ijms22115962
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发表时间：
2021-05-31
影响因子：
5.6
通讯作者：
Chen LQ
中科院分区：
文献类型：
--
作者：
Zhu L;Zhang XQ;Ye D;Chen LQ
植物特异性抗霉基因座O（MLO）蛋白含有7个跨膜结构域和1个保守的钙调素结合结构域，在植物的许多发育过程中发挥重要作用。然而，它们调节植物发育的机制仍不清楚。在这里，我们报告的MLO 4蛋白在拟南芥根的功能特性。在利用酵母双杂交（Y2 H）分析筛选来自拟南芥MLO和钙调素/钙调素样（CaM/CML）家族的蛋白质之间的相互作用中，MLO 4被鉴定为与CML 12相互作用。然后，通过荧光素酶互补成像（LCI）和双分子荧光互补（BiFC）测定进一步验证MLO 4和CML 12之间的相互作用。遗传分析表明，mlo 4，cml 12，和mlo 4 cml 12突变体表现出类似的缺陷，根重力反应。这些结果表明，MLO 4可能通过与CML 12的相互作用在根系重力响应中发挥重要作用。此外，我们的研究结果还表明，MLO和CaM/CML家族之间的相互作用可能是保守的。
The plant-specific mildew resistance locus O (MLO) proteins, which contain seven transmembrane domains and a conserved calmodulin-binding domain, play important roles in many plant developmental processes. However, their mechanisms that regulate plant development remain unclear. Here, we report the functional characterization of the MLO4 protein in Arabidopsis roots. The MLO4 was identified as interacting with CML12 in a screening for the interaction between the proteins from Arabidopsis MLO and calmodulin/calmodulin-like (CaM/CML) families using yeast two hybrid (Y2H) assays. Then, the interaction between MLO4 and CML12 was further verified by Luciferase Complementation Imaging (LCI) and Bimolecular Fluorescence Complementation (BiFC) assays. Genetic analysis showed that the mlo4, cml12, and mlo4 cml12 mutants displayed similar defects in root gravity response. These results imply that the MLO4 might play an important role in root gravity response through interaction with CML12. Moreover, our results also demonstrated that the interaction between the MLO and CaM/CML families might be conservative.
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影响因子：
12.3
作者：
Day IS;Reddy VS;Shad Ali G;Reddy AS
通讯作者：
Reddy AS
影响因子：
11.6
作者：
Chen, Zhongying;Noir, Sandra;Jones, Alan M.
通讯作者：
Jones, Alan M.
DOI：
10.1111/tpj.12639
发表时间：
2014-11
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Gookin TE;Assmann SM
通讯作者：
Assmann SM
影响因子：
30.8
作者：
Consonni, Chiara;Humphry, Matthew E.;Panstruga, Ralph
通讯作者：
Panstruga, Ralph
影响因子：
4.8
作者：
Dobney, Stephanie;Chiasson, David;Snedden, Wayne A.
通讯作者：
Snedden, Wayne A.