Evaluation and Genetic Analysis of Parthenocarpic Germplasms in Cucumber.
Evaluation and Genetic Analysis of Parthenocarpic Germplasms in Cucumber.
复制标题
黄瓜单性结实种质的评价和遗传分析。
作者：
Gou C;Zhu P;Meng Y;Yang F;Xu Y;Xia P;Chen J;Li J
单性结实是黄瓜（Cucumis sativus L.）生产然而，从国家黄瓜基因库中系统鉴定单性结实种质资源用于黄瓜改良仍然是一个国际性挑战。本研究对201个黄瓜品系进行了调查，包括不同的生态类型。在三个试验中评价了单性结实坐果（PFS）和单性结实果实扩展（PFE）的百分比。在自然群体中，PFS率符合正态分布，而PFE率呈偏态分布，表明PFS和PFE率都是典型的数量性状。遗传分析表明，单性结实性在不同生态型中均以相似的不完全显性方式遗传。对31个黄瓜品系进行单性结实全基因组关联分析，共检测到5324个与单性结实相关的单核苷酸多态性（single nucleotide polymorphisms，SNPs），从中鉴定出6个单性结实位点，包括2个新位点（Pfs1. 1和Pfs4. 1）。结果表明，15个黄瓜优良品系表现出较强的单性结实能力（PFS > 90%，PFE> 50%），其中6个黄瓜品系（18007s、18008s、18022s、18076s、18099s和18127s）表现出较弱的初果抑制。筛选出3个具有超子房单性结实的品系（18011s、18018s和18019s），表现出较好的吸引力。鉴定出4个低温强化单性结实品系（18018s、18022s、18029s和18012s），适合于反季节生产育种。我们的方法可以帮助提高效率，并导致现代黄瓜品种单性结实的改善。
Parthenocarpy is an important agronomic trait in cucumber (Cucumis sativus L.) production. However, the systematic identification of parthenocarpic germplasms from national gene banks for cucumber improvement remains an international challenge. In this study, 201 cucumber lines were investigated, including different ecotypes. The percentages of parthenocarpic fruit set (PFS) and parthenocarpic fruit expansion (PFE) were evaluated in three experiments. In natural populations, the PFS rates fit a normal distribution, while PFE rates showed a skewed distribution, suggesting that both PFS and PFE rates are typical quantitative traits. Genetic analysis showed that parthenocarpy in different ecotypes was inherited in a similar incompletely dominant manner. A total of 5324 single nucleotide polymorphisms (SNPs) associated with parthenocarpy were detected in a Genome-wide association study (GWAS) of parthenocarpy in the 31 cucumber lines, from which six parthenocarpic loci, including two novel loci (Pfs1.1 and Pfs4.1), were identified. Consequently, fifteen of the elite lines that were screened presented relatively stronger parthenocarpy ability (PFS > 90%, PFE > 50%), among which six cucumber lines (18007s, 18008s, 18022s, 18076s, 18099s, and 18127s) exhibited weak first-fruit inhibition. Three lines (18011s, 18018s, and 18019s) were screened for super ovary parthenocarpy, which showed more attractive performance. Four low-temperature-enhanced parthenocarpy lines (18018s, 18022s, 18029s, and 18012s) were identified, which were suited for breeding for counter-season production. Our approaches could help increase efficiency and lead to parthenocarpy improvements for modern cucumber cultivars.
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