Wisteria floribunda agglutinin-binding glycan expression is decreased in endometriomata.
Wisteria floribunda agglutinin-binding glycan expression is decreased in endometriomata.
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DOI：
10.1186/1477-7827-12-100
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发表时间：
2014-10-24
期刊：
影响因子：
--
通讯作者：
Narahara H
中科院分区：
文献类型：
--
作者：
Hirakawa T;Nasu K;Kai K;Aoyagi Y;Ishii T;Uemura T;Yano M;Narahara H
糖基化是真核生物蛋白质最常见的翻译后修饰之一，并且已知在广泛的生物过程中经历动态变化。然而，到目前为止，在子宫内膜异位症的聚糖表达谱在很大程度上是未知的。该研究的目的是确定在子宫内膜异位症（一种子宫内膜依赖性疾病）中异常表达的聚糖组。应用凝集素微阵列技术检测原代培养的人子宫内膜异位囊肿基质细胞（ECSC）和正常子宫内膜基质细胞（NESC）的糖蛋白表达谱。采用凝集素组织化学方法检测花紫藤凝集素（WFA）结合聚糖在卵巢囊肿和在位增生期子宫内膜中的分布。在ECSC和NESC中评估合成WFA结合聚糖的N-乙酰半乳糖胺转移酶的表达。我们发现，在ECSC中，WFA结合聚糖的水平降低。凝集素组织化学显示，WFA结合聚糖减少，只有在卵巢囊肿的基质成分，但不是在上皮成分，相比，在位增殖期子宫内膜。ECSC中合成WFA结合聚糖的N-乙酰半乳糖胺转移酶的表达下调。利用凝集素微阵列分析和凝集素组织化学，我们发现WFA结合聚糖减少子宫内膜异位症。在ECSC中，WFA结合聚糖的合成酶显著下调。这表明ECSC上具有WFA结合特性的N-聚糖表达减少是子宫内膜异位症的一个新特征。
Glycosylation is one of the most common post-translational modifications of eukaryotic proteins and is known to undergo dynamic changes in a wide range of biological processes. To date, however, the glycan expression profiles in endometriosis are largely unknown. The objective of the study was to identify the panel of glycans that were aberrantly expressed in endometriosis, a hormone-dependent disease. The glycan expression profiles in primary cultured human endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs) were determined by lectin microarray analysis. Distribution of Wisteria floribunda agglutinin (WFA)-binding glycans in ovarian endometriotic cysts and eutopic proliferative phase endometrium were assessed by lectin histochemistry. The expressions of N-acetylgalactosaminyl transferases that synthesize WFA-binding glycans were evaluated in ECSCs and NESCs. We found that the levels of WFA-binding glycans were decreased in ECSCs. Lectin histochemistry revealed that WFA-binding glycans were decreased only in the stromal components of the ovarian endometriotic cysts, but not in the epithelial components, compared to the eutopic proliferative phase endometrium. The expressions of N-acetylgalactosaminyl transferases that synthesize WFA-binding glycans were downregulated in ECSCs. Utilizing lectin microarray analysis and lectin histochemistry, we found that WFA-binding glycans were decreased in endometriosis. The synthetic enzymes of WFA-binding glycans were significantly downregulated in ECSCs. It is suggested that reduced expression of N-glycans with WFA-binding properties on ECSCs is a novel characteristics of endometriosis.
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影响因子：
3.1
作者：
Dall'Olio, Fabio;Malagolini, Nadia;Chiricolo, Mariella
通讯作者：
Chiricolo, Mariella
DOI：
10.1016/j.ejogrb.2013.08.008
发表时间：
2013-11-01
影响因子：
2.6
作者：
Berkes, E.;Muzinic, A.;Oehmke, F.
通讯作者：
Oehmke, F.
影响因子：
11.2
作者：
Napoletano, Chiara;Rughetti, Aurelia;Nuti, Marianna
通讯作者：
Nuti, Marianna
影响因子：
5.2
作者：
Huang, John;Liang, Jin-Tung;Huang, Min-Chuan
通讯作者：
Huang, Min-Chuan
影响因子：
6
作者：
Hsu, Wen-Ming;Che, Mei-leng;Huang, Min-Chuan
通讯作者：
Huang, Min-Chuan