Image-based closed-loop feedback for highly mono-dispersed microdroplet production.
Image-based closed-loop feedback for highly mono-dispersed microdroplet production.
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DOI：
10.1038/s41598-017-11254-5
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发表时间：
2017-09-05
影响因子：
4.6
通讯作者：
Whyte G
中科院分区：
文献类型：
--
作者：
Crawford DF;Smith CA;Whyte G
由不混溶液体分离出的微米级液滴可以提供可在微流体网络中操纵的小型化反应容器，并且发展迅速。在许多实验中，这些微滴的精确体积是一个关键参数，可能受到许多外部因素的影响。在这项工作中，我们展示了基于成像的反馈和压力驱动泵的结合，以精确控制微流体装置中产生的微滴的尺寸。使用快速响应、压力驱动泵可以快速、准确地改变微流体流动，同时直接测量液滴尺寸允许用户定义更有意义的参数，即液滴尺寸和生成频率，而不是流速或压力。反馈回路能够对基于压力的泵进行漂移校正，并导致长时间产生的液滴的单分散性大幅增加。我们还展示了如何将其扩展到控制多个液体流，从而允许控制液滴形成的频率或每个液滴的活细胞的平均浓度并保持恒定。
Micron-scale droplets isolated by an immiscible liquid can provide miniaturised reaction vessels which can be manipulated in microfluidic networks, and has seen a rapid growth in development. In many experiments, the precise volume of these microdroplets is a critical parameter which can be influenced by many external factors. In this work, we demonstrate the combination of imaging-based feedback and pressure driven pumping to accurately control the size of microdroplets produced in a microfluidic device. The use of fast-response, pressure-driving pumps allows the microfluidic flow to be quickly and accurately changed, while directly measuring the droplet size allows the user to define the more meaningful parameters of droplet size and generation frequency rather than flow rates or pressures. The feedback loop enables the drift correction of pressure based pumps, and leads to a large increase in the mono-dispersity of the droplets produced over long periods. We also show how this can be extended to control multiple liquid flows, allowing the frequency of droplet formation or the average concentration of living cells per droplet to be controlled and kept constant.
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影响因子：
14.8
作者：
Mazutis, Linas;Gilbert, John;Ung, W. Lloyd;Weitz, David A.;Griffiths, Andrew D.;Heyman, John A.
通讯作者：
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影响因子：
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影响因子：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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