Methicillin resistance transfer from Staphylocccus epidermidis to methicillin-susceptible Staphylococcus aureus in a patient during antibiotic therapy.
Methicillin resistance transfer from Staphylocccus epidermidis to methicillin-susceptible Staphylococcus aureus in a patient during antibiotic therapy.
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DOI：
10.1371/journal.pone.0011841
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发表时间：
2010-07-29
期刊：
影响因子：
3.7
通讯作者：
Fluit AC
中科院分区：
文献类型：
--
作者：
Bloemendaal AL;Brouwer EC;Fluit AC
编码葡萄球菌甲氧西林耐药性的mecA基因位于称为葡萄球菌盒式染色体mec（SCCmec）的移动的遗传元件上。这种元素的水平种间转移可能是耐甲氧西林沙门氏菌传播的重要因素。金黄色葡萄球菌（MRSA）。以前，我们报告了一个密切相关的甲氧西林敏感金黄色葡萄球菌（MSSA），MRSA和潜在的SCCmec供体表皮葡萄球菌分离自同一患者的分离。基于指纹技术，我们假设S。表皮葡萄球菌已将SCCmec转移到MSSA，成为MRSA。本研究的目的是表明，这些菌株形成一个同基因对，种间水平SCCmec转移发生。对两种分离株进行全基因组测序，并通过常规测序关闭MSSA缺口。S.还通过常规方法对epidermidis进行测序。结果显示，除了MRSA中存在SCCmec外，两种分离株之间的核苷酸序列没有差异。S.表皮葡萄球菌和MRSA是相同的，除了ccrB基因中的单个核苷酸，其导致缬氨酸到丙氨酸的取代。与密切相关的EMRSA-16的主要区别是在MSSA-MRSA对中存在编码中毒性休克综合征毒素和剥脱性毒素A的SaPI 2。没有SCCmec从S.表皮与MSSA的关系可以在体外得到证实。除SCCmec外，MSSA和MRSA形成同基因对。这有力地支持了我们的假设，即MRSA是通过SCCmec从S. epidermidis O7.1.
The mecA gene, encoding methicillin resistance in staphylococci, is located on a mobile genetic element called Staphylococcal Cassette Chromosome mec (SCCmec). Horizontal, interspecies transfer of this element could be an important factor in the dissemination of methicillin-resistant S. aureus (MRSA). Previously, we reported the isolation of a closely related methicillin-susceptible Staphylococcus aureus (MSSA), MRSA and potential SCCmec donor Staphylococcus epidermidis isolate from the same patient. Based on fingerprint techniques we hypothesized that the S. epidermidis had transferred SCCmec to the MSSA to become MRSA. The aim of this study was to show that these isolates form an isogenic pair and that interspecies horizontal SCCmec transfer occurred. Whole genome sequencing of both isolates was performed and for the MSSA gaps were closed by conventional sequencing. The SCCmec of the S. epidermidis was also sequenced by conventional methods. The results show no difference in nucleotide sequence between the two isolates except for the presence of SCCmec in the MRSA. The SCCmec of the S. epidermidis and the MRSA are identical except for a single nucleotide in the ccrB gene, which results in a valine to alanine substitution. The main difference with the closely related EMRSA-16 is the presence of SaPI2 encoding toxic shock syndrome toxin and exfoliative toxin A in the MSSA-MRSA pair. No transfer of SCCmec from the S. epidermidis to the MSSA could be demonstrated in vitro. The MSSA and MRSA form an isogenic pair except for SCCmec. This strongly supports our hypothesis that the MRSA was derived from the MSSA by interspecies horizontal transfer of SCCmec from S. epidermidis O7.1.
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影响因子：
14.2
作者：
Berglund, C.;Soderquist, B.
通讯作者：
Soderquist, B.
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